KFU. Card publication. In Situ functionalization of Poly(hydroxyethyl methacrylate) Cryogels with Oligopeptides via β-Cyclodextrin–Adamantane Complexation for Studying Cell-Instructive Peptide Environment

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IN SITU FUNCTIONALIZATION OF POLY(HYDROXYETHYL METHACRYLATE) CRYOGELS WITH OLIGOPEPTIDES VIA β-CYCLODEXTRIN&NDASH;ADAMANTANE COMPLEXATION FOR STUDYING CELL-INSTRUCTIVE PEPTIDE ENVIRONMENT

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Zukhayb Mokhamed , author

Bibliographic description in the original language

Luong T.D. In Situ functionalization of Poly(hydroxyethyl methacrylate) Cryogels with Oligopeptides via β-Cyclodextrin–Adamantane Complexation for Studying Cell-Instructive Peptide Environment / T. D. Luong, M. Zoughaib, R. Garifullin, S. Kuznetsova, M.O. Guler, T.I. Abdullin // ACS Appl. Bio Mater. - 2020. - � 3. -c. 1116-1128

Annotation

ACS Applied Bio Materials

Keywords

bioactive peptide motifs, extracellular matrix, growth factors, poly(hydroxyethyl methacrylate) cryogels, cyclodextrin-adamantane inclusion complex, RGD, GHK, affinity immobilization, cell responses

The name of the journal

ACS Applied Bio Materials

URL

https://pubs.acs.org/doi/abs/10.1021/acsabm.9b01059

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=236605&p_lang=2

Resource files

File name	Size (MB)	Format
In_Situ_functionalization_of_Poly_hydroxyethyl_methacrylate_.pdf	7,22	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Zukhayb Mokhamed	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Luong T.D. In Situ functionalization of Poly(hydroxyethyl methacrylate) Cryogels with Oligopeptides via β-Cyclodextrin–Adamantane Complexation for Studying Cell-Instructive Peptide Environment / T. D. Luong, M. Zoughaib, R. Garifullin, S. Kuznetsova, M.O. Guler, T.I. Abdullin // ACS Appl. Bio Mater. - 2020. - � 3. -c. 1116-1128	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=236605&p_lang=2	ru_RU
dc.description.abstract	ACS Applied Bio Materials	ru_RU
dc.description.abstract	Oligopeptides are versatile cell modulators resembling pleiotropic activities of ECM proteins and growth factors. Studying the role of cell-instructive peptide signals within 3D scaffolds, yet poorly known, requires effective approaches to introducing bioactive sequences into appropriate materials. We synthesized RGD and GHK motif based peptides 1 and 2 linked to the terminal adamantyl group (Ad) and their fluorescent derivatives 3 and 4. Poly(hydroxyethyl methacrylate) (pHEMA) cryogels with additional PEG/β-cyclodextrin (CD) units were prepared as an inert macroporous scaffold capable to bind the adamantylated peptides via affinity CD-Ad complexation. According to toluidine blue staining, the CD moieties were effectively and stably incorporated in the pHEMA cryogels at nanomolar amounts per milligram of material. The CD component gradually increased the thickness and swelling ability of the polymer walls of cryogels, resulting in a noticeable decrease in macropore size and modulation of viscoelastic properties. The labeled peptides exhibited fast kinetics of specific binding to the CD-modified cryogels and were simultaneously immobilized by coincubation. The peptide loading approached ca. 0.31 mg per cm2 of cryogel sheet. A well-defined mitogenic effect of the immobilized peptides (2 < 1≪ 1 + 2) was revealed toward 3T3 and PC-12 cells. The synergistic action of RGD and GHK peptides induced a profound change in cell behavior/morphology attributed to a growth-factor-like activity of the composition. Altogether, our results provide an effective procedure for the preparation of CD-modified pHEMA cryogels and their uniform in situ functionalization with bioactive peptide(s) of interest and an informative study of cellular responses in the functionalized scaffolds.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	bioactive peptide motifs	ru_RU
dc.subject	extracellular matrix	ru_RU
dc.subject	growth factors	ru_RU
dc.subject	poly(hydroxyethyl methacrylate) cryogels	ru_RU
dc.subject	cyclodextrin-adamantane inclusion complex	ru_RU
dc.subject	RGD	ru_RU
dc.subject	GHK	ru_RU
dc.subject	affinity immobilization	ru_RU
dc.subject	cell responses	ru_RU
dc.title	In Situ functionalization of Poly(hydroxyethyl methacrylate) Cryogels with Oligopeptides via β-Cyclodextrin–Adamantane Complexation for Studying Cell-Instructive Peptide Environment	ru_RU
dc.type	Articles in international journals and collections	ru_RU