KFU. Card publication. Spoligotyping of tuberculous mycobacteria isolated from humans and cattle

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

SPOLIGOTYPING OF TUBERCULOUS MYCOBACTERIA ISOLATED FROM HUMANS AND CATTLE

Form of presentation

Articles in international journals and collections

Year of publication

2020

Язык

английский

Authors, employees of KFU

Shuralev Eduard Arkadevich, author

Other authors

Osyanin K. A., author

Faizov T. Kh., author

Khaertynov K. S., author

Khammadov N. I., author

Authors, students KFU

Khammadova Alfiya Vasilevna, author

Bibliographic description in the original language

Khammadov N.I, Faizov T.K, Osyanin K.A, Khammadova A.V., Khaertynov K.S., Shuralev E.A. Spoligotyping of tuberculous mycobacteria isolated from humans and cattle // Tuberculosis and Lung Diseases. - 2020. - Vol.98, Is.3. - P. 13-18. doi.org/10.21292/2075-1230-2020-98-3-13-18

Annotation

The article assesses the variability of sites within direct repeats (spacers), which are used in laboratory diagnostics for spoligotyping of mycobacteria and identification of tuberculosis pathogens. The frequency of combinations of different spacers in the analyzed mycobacteria in the specimens identified as Mycobacterium tuberculosis complex is compared with the spoligotyping profile of known mycobacteria thus establishing their belonging to a specific genetic family. The studied isolates were tested by microscopic, bacteriological, and molecular genetic (PCR) methods. Based on the results of spoligotyping, it was found out that they belonged to genetic families of M. tuberculosis of Beijing, LAM, and Haarlem.

Keywords

Mycobacterium bovis, Mycobacterium tuberculosis, tuberculosis, human, cattle, species, PCR, spoligotyping

The name of the journal

Tuberculosis and Lung Diseases

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085599720&doi=10.21292%2f2075-1230-2020-98-3-13-18&partnerID=40&md5=3228610858c709eb0559e03b100406e6

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=235132&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Shuralev Eduard Arkadevich	ru_RU
dc.contributor.author	Osyanin K. A.	ru_RU
dc.contributor.author	Faizov T. Kh.	ru_RU
dc.contributor.author	Khaertynov K. S.	ru_RU
dc.contributor.author	Khammadov N. I.	ru_RU
dc.contributor.author	Khammadova Alfiya Vasilevna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Khammadov N.I, Faizov T.K, Osyanin K.A, Khammadova A.V., Khaertynov K.S., Shuralev E.A. Spoligotyping of tuberculous mycobacteria isolated from humans and cattle // Tuberculosis and Lung Diseases. - 2020. - Vol.98, Is.3. - P. 13-18. doi.org/10.21292/2075-1230-2020-98-3-13-18	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=235132&p_lang=2	ru_RU
dc.description.abstract	Tuberculosis and Lung Diseases	ru_RU
dc.description.abstract	The article assesses the variability of sites within direct repeats (spacers), which are used in laboratory diagnostics for spoligotyping of mycobacteria and identification of tuberculosis pathogens. The frequency of combinations of different spacers in the analyzed mycobacteria in the specimens identified as Mycobacterium tuberculosis complex is compared with the spoligotyping profile of known mycobacteria thus establishing their belonging to a specific genetic family. The studied isolates were tested by microscopic, bacteriological, and molecular genetic (PCR) methods. Based on the results of spoligotyping, it was found out that they belonged to genetic families of M. tuberculosis of Beijing, LAM, and Haarlem.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Mycobacterium bovis	ru_RU
dc.subject	Mycobacterium tuberculosis	ru_RU
dc.subject	tuberculosis	ru_RU
dc.subject	human	ru_RU
dc.subject	cattle	ru_RU
dc.subject	species	ru_RU
dc.subject	PCR	ru_RU
dc.subject	spoligotyping	ru_RU
dc.title	Spoligotyping of tuberculous mycobacteria isolated from humans and cattle	ru_RU
dc.type	Articles in international journals and collections	ru_RU