KFU. Card publication. Preferential solvation of lysozyme in water-dimethyl sulfoxide mixtures: Gibbs energies of water and organic solvent.

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FEDERAL UNIVERSITY

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PREFERENTIAL SOLVATION OF LYSOZYME IN WATER-DIMETHYL SULFOXIDE MIXTURES: GIBBS ENERGIES OF WATER AND ORGANIC SOLVENT.

Form of presentation

Articles in international journals and collections

Year of publication

2019

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Authors, employees of KFU

Sirotkin Vladimir Aleksandrovich, author

Bibliographic description in the original language

Sirotkin, V.A. Preferential solvation of lysozyme in water-dimethyl sulfoxide mixtures: Gibbs energies of water and organic solvent. / In: Sirotkin V.A., (Ed.) Preferential solvation and hydration of proteins in water-organic mixtures: Two sides of one coin. - Nova Science Publishers, Inc., Hauppauge, NY, 2019. - P.91-122.

Annotation

Preferential solvation and hydration of proteins in water-organic mixtures: Two sides of one coin.

Keywords

Preferential solvation, lysozyme, water-dimethyl sulfoxide mixtures, Gibbs energies, water, organic solvent

The name of the journal

Preferential solvation and hydration of proteins in water-organic mixtures: Two sides of one coin.

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084275481&partnerID=40&md5=b603c8f11abb363f1985ace6d00f7ed2

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=233978&p_lang=2

Resource files

File name	Size (MB)	Format
Book_2019_Chapter_04.pdf	0,48	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sirotkin Vladimir Aleksandrovich	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Sirotkin, V.A. Preferential solvation of lysozyme in water-dimethyl sulfoxide mixtures: Gibbs energies of water and organic solvent. / In: Sirotkin V.A., (Ed.) Preferential solvation and hydration of proteins in water-organic mixtures: Two sides of one coin. - Nova Science Publishers, Inc., Hauppauge, NY, 2019. - P.91-122.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=233978&p_lang=2	ru_RU
dc.description.abstract	Preferential solvation and hydration of proteins in water-organic mixtures: Two sides of one coin.	ru_RU
dc.description.abstract	Water/organic solvent sorption and residual enzyme activity were investigated to monitor preferential solvation and preferential hydration of protein macromolecules in the entire range of water content in organic liquids at 25?C. This approach was applied to estimate protein destabilization/stabilization due to the preferential interactions of hen-egg-white lysozyme with water-dimethyl sulfoxide (DMSO) mixtures. This approach facilitates the individual evaluation of the Gibbs energies of water, protein, and organic solvent. There are two concentration regimes. Lysozyme is preferentially hydrated at high water content. The residual enzyme activity values are close to 100%. At low water content, lysozyme has a higher affinity for DMSO than for water. The DMSO-induced irreversible inactivation of α-chymotrypsin was found in this region. Residual enzyme activity is close to zero in this concentration range.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Preferential solvation	ru_RU
dc.subject	lysozyme	ru_RU
dc.subject	water-dimethyl sulfoxide mixtures	ru_RU
dc.subject	Gibbs energies	ru_RU
dc.subject	water	ru_RU
dc.subject	organic solvent	ru_RU
dc.title	Preferential solvation of lysozyme in water-dimethyl sulfoxide mixtures: Gibbs energies of water and organic solvent.	ru_RU
dc.type	Articles in international journals and collections	ru_RU