KFU. Card publication. Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors

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ACETYLCHOLINESTERASE SENSOR BASED ON POLYELECTROLYTE COMPLEXES WITH DNA INCLUSION FOR THE DETERMINATION OF REVERSIBLE INHIBITORS

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Evtyugin Gennadiy Arturovich, author

Ivanov Aleksey Nikolaevich, author

Authors, postgraduates KFU

Davletshina Regina Rudisovna, postgraduate kfu

Bibliographic description in the original language

R. Davletshina, A. Ivanov, G. Evtugyn, Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors // Electroanalysis. - 2020. - V.32, No 2. - P. 308-316.

Annotation

The acetylcholinesterase (AChE) biosensor has been developed for the determination of reversible inhibitors applied in the Alzheimer's disease therapy, i. e., Huperzine A (HupA) and galantamine (Gal). For this purpose, glassy carbon electrode (GCE) was first modified with carbon black (CB) and Co phthalocyanine and then polyelectrolyte complex was self‐assembled on its surface by drop casting of reactants and washing. To extend the stability and improve biosensor performance, it was proposed for the first time to use DNA as polyanion in the complex assembly. The DNA showed higher charge density than conventional polyelectrolytes and stabilized the surface coating by adsorption of higher enzyme amount and prevention of its leaching during the biosensor operation. Complex formation and the influence of structural factors were monitored with surface plasmon resonance. Kinetic study showed mixed inhibition of the enzyme within micro‐ and nanomolar range of inhibitor concentrations.

Keywords

acetylcholinesterase biosensor, reversible inhibition, polyelectrolyte complex, Alzheimer's disease drug

The name of the journal

ELECTROANALYSIS

URL

https://doi.org/10.1002/elan.201900507

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=218766&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Evtyugin Gennadiy Arturovich	ru_RU
dc.contributor.author	Ivanov Aleksey Nikolaevich	ru_RU
dc.contributor.author	Davletshina Regina Rudisovna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	R. Davletshina, A. Ivanov, G. Evtugyn, Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors // Electroanalysis. - 2020. - V.32, No 2. - P. 308-316.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=218766&p_lang=2	ru_RU
dc.description.abstract	ELECTROANALYSIS	ru_RU
dc.description.abstract	The acetylcholinesterase (AChE) biosensor has been developed for the determination of reversible inhibitors applied in the Alzheimer's disease therapy, i. e., Huperzine A (HupA) and galantamine (Gal). For this purpose, glassy carbon electrode (GCE) was first modified with carbon black (CB) and Co phthalocyanine and then polyelectrolyte complex was self‐assembled on its surface by drop casting of reactants and washing. To extend the stability and improve biosensor performance, it was proposed for the first time to use DNA as polyanion in the complex assembly. The DNA showed higher charge density than conventional polyelectrolytes and stabilized the surface coating by adsorption of higher enzyme amount and prevention of its leaching during the biosensor operation. Complex formation and the influence of structural factors were monitored with surface plasmon resonance. Kinetic study showed mixed inhibition of the enzyme within micro‐ and nanomolar range of inhibitor concentrations.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	acetylcholinesterase biosensor	ru_RU
dc.subject	reversible inhibition	ru_RU
dc.subject	polyelectrolyte complex	ru_RU
dc.subject	Alzheimer's disease drug	ru_RU
dc.title	Acetylcholinesterase Sensor Based on Polyelectrolyte Complexes with DNA inclusion for the Determination of Reversible Inhibitors	ru_RU
dc.type	Articles in international journals and collections	ru_RU