KFU. Card publication. Analysis of autophagy and LC3-associated phagocytosis in T-lymphocytes of patients with severe asthma

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ANALYSIS OF AUTOPHAGY AND LC3-ASSOCIATED PHAGOCYTOSIS IN T-LYMPHOCYTES OF PATIENTS WITH SEVERE ASTHMA

Form of presentation

Articles in international journals and collections

Year of publication

2019

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Authors, employees of KFU

Abramov Sergey Nikolaevich, author

Abramova Zinaida Ivanovna, author

Skibo Yuliya Valerevna, author

Tikhomirova Mariya Vladimirovna, author

Bibliographic description in the original language

Abramov S. N. P352 Analysis of autophagy and LC3-associated phagocytosis in T-lymphocytes of patients with severe asthma/S. N. Abramov, Y. V. Skibo, A. Z. Abramova, M. V. Tihomirova //Human Gene Therapy,-2019.-V. 30, No. 11.-A120-A121

Annotation

A lot of diseases are characterized by a dysfunction of apoptosis and immune activity, although one process is distinguished as a link between the appearance of autoantigens and impaired immune tolerance. Autophagy is a process of intracellular degradation, which is aimed at maintaining the cell homeostasis by delivering the cytoplasmic components to lysosomes. According to the studies, the greatest interest in the context of human diseases represents the non-canonical form of autophagy – LC3-associated phagocytosis (or LAP). For years, it has been assumed that asthma and autoimmune diseases have little in common, however, both of them are characterized by involving the immune system components, and in case of asthma, the autoimmune component. Thus, we performed a comprehensive ultrastructural analysis of apoptosis, autophagy and LAP in patients with severe bronchial asthma. By using the transmission electron microscopy, we found that the majority of T-lymphocytes in the control group have a morphology that is common for the early and late stages of apoptosis. Autophagosomes were not detected, however, a significant number of single-membrane phagosomes associated with the LAP pathway were found. In patients with severe asthma, in addition to the signs of the early stages of apoptosis, increased content of autophagosomes and a small number of single-membrane phagosomes were detected. As a result, a weak manifestation of LC3-associated phagocytosis, and the inhibition of apoptosis in the final stages may cause the appearance of autoantigens and the development of an autoimmune response. Study was funded by RFBR according to the research project �18-34-00739.

Keywords

autophagy, LC3-associated phagocytosis, T-lymphocytes, asthma

The name of the journal

HUMAN GENE THERAPY

URL

https://www.liebertpub.com/doi/10.1089/hum.2019.29095.abstracts

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=214950&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramov Sergey Nikolaevich	ru_RU
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.contributor.author	Skibo Yuliya Valerevna	ru_RU
dc.contributor.author	Tikhomirova Mariya Vladimirovna	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Abramov S. N. P352 Analysis of autophagy and LC3-associated phagocytosis in T-lymphocytes of patients with severe asthma/S. N. Abramov, Y. V. Skibo, A. Z. Abramova, M. V. Tihomirova //Human Gene Therapy,-2019.-V. 30, No. 11.-A120-A121	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=214950&p_lang=2	ru_RU
dc.description.abstract	HUMAN GENE THERAPY	ru_RU
dc.description.abstract	A lot of diseases are characterized by a dysfunction of apoptosis and immune activity, although one process is distinguished as a link between the appearance of autoantigens and impaired immune tolerance. Autophagy is a process of intracellular degradation, which is aimed at maintaining the cell homeostasis by delivering the cytoplasmic components to lysosomes. According to the studies, the greatest interest in the context of human diseases represents the non-canonical form of autophagy – LC3-associated phagocytosis (or LAP). For years, it has been assumed that asthma and autoimmune diseases have little in common, however, both of them are characterized by involving the immune system components, and in case of asthma, the autoimmune component. Thus, we performed a comprehensive ultrastructural analysis of apoptosis, autophagy and LAP in patients with severe bronchial asthma. By using the transmission electron microscopy, we found that the majority of T-lymphocytes in the control group have a morphology that is common for the early and late stages of apoptosis. Autophagosomes were not detected, however, a significant number of single-membrane phagosomes associated with the LAP pathway were found. In patients with severe asthma, in addition to the signs of the early stages of apoptosis, increased content of autophagosomes and a small number of single-membrane phagosomes were detected. As a result, a weak manifestation of LC3-associated phagocytosis, and the inhibition of apoptosis in the final stages may cause the appearance of autoantigens and the development of an autoimmune response. Study was funded by RFBR according to the research project �18-34-00739.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	autophagy	ru_RU
dc.subject	LC3-associated phagocytosis	ru_RU
dc.subject	T-lymphocytes	ru_RU
dc.subject	asthma	ru_RU
dc.title	Analysis of autophagy and LC3-associated phagocytosis in T-lymphocytes of patients with severe asthma	ru_RU
dc.type	Articles in international journals and collections	ru_RU