KFU. Card publication. Role of microvesicles derived from adipose mesenchymal stem cells in liver regeneration after partial hepatectomy in rats

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ROLE OF MICROVESICLES DERIVED FROM ADIPOSE MESENCHYMAL STEM CELLS IN LIVER REGENERATION AFTER PARTIAL HEPATECTOMY IN RATS

Form of presentation

Conference proceedings in international journals and collections

Year of publication

2019

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Authors, employees of KFU

Bilyalov Ayrat Ildarovich, author

Gomzikova Marina Olegovna, author

Gumerova Anisa Azatovna, author

Kiyasov Andrey Pavlovich, author

Rizvanov Albert Anatolevich, author

Titova Angelina Andreevna, author

Shafigullina Aygul Kasyymovna, author

Authors, students KFU

Barkov Andrey Yurevich, author

Kundakchyan Grayr Grayrovich, author

Usmonova Gulbakhor Okilzhon Kizi, author

Bibliographic description in the original language

Shafigullina A.K. Role of microvesicles derived from adipose mesenchymal stem cells in liver regeneration after partial hepatectomy in rats / Shafigullina A.K., Gomzikova M.O., Titova A.A., Barkov A.Y., Kundakchyan G.G., Usmonova G.O., Bilyalov A., Gumerova A.A., Rizvanov A.A., Kiyasov A.P. // United European Gastroenterology Week, October 19-23, 2019, Barcelona, Spain. – P0696. – P.466.

Annotation

United European Gastroenterology Week Journal

Keywords

microvesicles, adipose mesenchymal stem cells, liver regeneration, partial hepatectomy

The name of the journal

United European Gastroenterology Week Journal

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/151975/tezisy_UEG_2019.pdf?sequence=1&isAllowed=y

URL

https://www.ueg.eu/journal/

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=213544&p_lang=2

Resource files

File name	Size (MB)	Format
tezisy_UEG_2019.pdf	0,08	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Bilyalov Ayrat Ildarovich	ru_RU
dc.contributor.author	Gomzikova Marina Olegovna	ru_RU
dc.contributor.author	Gumerova Anisa Azatovna	ru_RU
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.contributor.author	Shafigullina Aygul Kasyymovna	ru_RU
dc.contributor.author	Barkov Andrey Yurevich	ru_RU
dc.contributor.author	Kundakchyan Grayr Grayrovich	ru_RU
dc.contributor.author	Usmonova Gulbakhor Okilzhon Kizi	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Shafigullina A.K. Role of microvesicles derived from adipose mesenchymal stem cells in liver regeneration after partial hepatectomy in rats / Shafigullina A.K., Gomzikova M.O., Titova A.A., Barkov A.Y., Kundakchyan G.G., Usmonova G.O., Bilyalov A., Gumerova A.A., Rizvanov A.A., Kiyasov A.P. // United European Gastroenterology Week, October 19-23, 2019, Barcelona, Spain. – P0696. – P.466.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=213544&p_lang=2	ru_RU
dc.description.abstract	United European Gastroenterology Week Journal	ru_RU
dc.description.abstract	Introduction. There are numerous studies of stimulation of liver regeneration by transplantation of mesenchymal stem cells. Their effect is explained by direct intercellular interactions and paracrine communications. One of the alternative ways to influence on liver regeneration is injection of microvesicles. Microvesicles are extracellular vesicles that contain growth factors and cytokines and play a major role in intercellular paracrine communications. It is said, that in compare to cells microvesicles transplantation is not accompanied by risk of metaplasia and mutations, because they do not contain any genetic information. To study the effect of transplantation of mesenchymal stem cells microvesicles derived from adipose fat tissue on liver regeneration after partial hepatectomy in rats. Methods. Mesenchymal stem cells, isolated from visceral rat fatty tissue (adMSC), were treated by Cytochalasin B to derive microvesicles. These microvesicles were transplanted into portal vein of rats after partial hepatectomy. Control group of rats after partial hepatectomy received injection of PBS. On 2, 5, 7 and 14 days after operation rats were sacrificed. Functional parameters of liver were analyzed by biochemical tests, morphological changes were studied by immunohistochemical staining of liver slices with antibodies to desmin (HSC marker), Ki-67 (proliferation marker), α-SMA (myofibroblasts marker), CK-19 (cholangiocytes marker). Results. According to immunohistochemistry results injection of microvesicles: 1) inhibits activation of HSC – there were 20% less desmin+ cells; 2) there were no transformation of HSC into α-SMA+ myofibroblasts and no risk of liver fibrosis; 3) inhibition of cellular proliferation. So, Ki-67+ hepatocytes number (area of portal tract and central vein) decreased in compare to control group. Number of Ki-67+ nonparenchymal cells in portal tract area was 2 times less, than in control. 4) there were no differences in CK-19 expression in experimental and control groups. As far as the cellular proliferation and thus liver regeneration was inhibited, we`ve seen higher numbers of ALT levels in experimental group. Decreased regeneration could be also visualized by lower triglycerides and cholesterol levels, there were less, than normal values. Blood urea nitrogen normalized in control group on 14th day, but in experimental – on 7th day already. Conclusion. Injection of adMSC microvesicles inhibits general cellular response to partial hepatectomy. Inhibition of hepatocytes activation and proliferation slows down liver regeneration, that is proved by biochemical tests. Inhibition of HSC activation means also lesser risk of their transformation into myofibroblasts and fibrosis development. Thus microvesicles transplantation is not for stimulation of liver regeneration. Probably these inhibitory effects could be applied for treatment of liver fibrosis, that needs to be studied. Work supported by Program of Competitive Growth of KFU.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	microvesicles	ru_RU
dc.subject	adipose mesenchymal stem cells	ru_RU
dc.subject	liver regeneration	ru_RU
dc.subject	partial hepatectomy	ru_RU
dc.title	Role of microvesicles derived from adipose mesenchymal stem cells in liver regeneration after partial hepatectomy in rats	ru_RU
dc.type	Conference proceedings in international journals and collections	ru_RU