KFU. Card publication. Cellular uptake and cytotoxicity of unmodified Pr3+:LaF 3 nanoparticles

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

CELLULAR UPTAKE AND CYTOTOXICITY OF UNMODIFIED PR3+:LAF 3 NANOPARTICLES

Form of presentation

Articles in international journals and collections

Year of publication

2019

Язык

английский

Authors, employees of KFU

Evtyugin Vladimir Gennadevich, author

Zelenikhin Pavel Valerevich, author

Nizamutdinov Aleksey Sergeevich, author

Pudovkin Maksim Sergeevich, author

Salnikov Vadim Vladimirovich, author

Semashko Vadim Vladimirovich, author

Other authors

Shtyreva Victoria , author

Bibliographic description in the original language

Pudovkin M.S. Cellular uptake and cytotoxicity of unmodified Pr3+:LaF 3 nanoparticles / Maksim S. Pudovkin, Pavel V. Zelenikhin, Victoria V. Shtyreva,Vladimir G. Evtugyn, Vadim V. Salnikov, Alexey S. Nizamutdinov, Vadim V. Semashko // J Nanopart Res.(2019) 21:184

Annotation

Pr3+:LaF 3 (C Pr = 1%) nanoparticles were characterized by means of transmission electron micros-copy (TEM), X-ray diffraction, energy-dispersive spec-troscopy, and optical spectroscopy. The obtained 14 nm Pr3+:LaF 3 (C Pr = 1%) crystalline hexagonal-structured nanoparticles contain Pr, La, and F only. The lumines-cent spectra emission bands corresponded to the emission bands of Pr3+ions. The Pr3+:LaF 3 (C Pr = 1%) nano-particles effectively interact with A 549, LE С , and MDCK cells. By means of TEM, it was revealed that after 2 h of the nanoparticle exposure, A 549, MDCK, and LE С cells internalized the nanoparticles and 20 –300 nm agglomerates of the nanoparticles packed into 200 – 500 nm vesicles were found into the cytoplasm. It seems that the internalization occurs via macropinocytosis. In A 549 cells, some vesicles were disrupted and the nanoparticles escaped the vesicles floating freely in the cytoplasm. Flow cytometry showed that all the cells effectively interact with nanoparticles. This interaction leads to cell granularity change. Specifically, A 549, MDCK, and LE С , and cells treated by nanoparticles have the values of size scattered signal 16 ? 2, 20 ? 3, and 39 ? 3%, respectively, com-paring with the untreated cells. The Pr3+:LaF 3 (C Pr = 1%) nanoparticles were not found into the cellular or-ganelles. The cytotoxicity of the Pr3+:LaF 3 (C Pr = 1%) nanoparticles is not significant at concentrations of 0.05,0.1, 0.25, and 0.5 g/L

Keywords

Pr3+:LaF 3 nanoparticles . Fluoride nanoparticles . Cytotoxicity. Cellular uptake . Flow cytometry. Macropinocytosis . Nanobiomedicine

The name of the journal

J NANOPART RES

URL

https://link.springer.com/article/10.1007/s11051-019-4628-9

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=208495&p_lang=2

Resource files

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F_Pudovkin_et_al_2019_Journal_of_Nanoparticle_Research.pdf	5,32	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Evtyugin Vladimir Gennadevich	ru_RU
dc.contributor.author	Zelenikhin Pavel Valerevich	ru_RU
dc.contributor.author	Nizamutdinov Aleksey Sergeevich	ru_RU
dc.contributor.author	Pudovkin Maksim Sergeevich	ru_RU
dc.contributor.author	Salnikov Vadim Vladimirovich	ru_RU
dc.contributor.author	Semashko Vadim Vladimirovich	ru_RU
dc.contributor.author	Shtyreva Victoria	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Pudovkin M.S. Cellular uptake and cytotoxicity of unmodified Pr3+:LaF 3 nanoparticles / Maksim S. Pudovkin, Pavel V. Zelenikhin, Victoria V. Shtyreva,Vladimir G. Evtugyn, Vadim V. Salnikov, Alexey S. Nizamutdinov, Vadim V. Semashko // J Nanopart Res.(2019) 21:184	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=208495&p_lang=2	ru_RU
dc.description.abstract	J NANOPART RES	ru_RU
dc.description.abstract	Pr3+:LaF 3 (C Pr = 1%) nanoparticles were characterized by means of transmission electron micros-copy (TEM), X-ray diffraction, energy-dispersive spec-troscopy, and optical spectroscopy. The obtained 14 nm Pr3+:LaF 3 (C Pr = 1%) crystalline hexagonal-structured nanoparticles contain Pr, La, and F only. The lumines-cent spectra emission bands corresponded to the emission bands of Pr3+ions. The Pr3+:LaF 3 (C Pr = 1%) nano-particles effectively interact with A 549, LE С , and MDCK cells. By means of TEM, it was revealed that after 2 h of the nanoparticle exposure, A 549, MDCK, and LE С cells internalized the nanoparticles and 20 –300 nm agglomerates of the nanoparticles packed into 200 – 500 nm vesicles were found into the cytoplasm. It seems that the internalization occurs via macropinocytosis. In A 549 cells, some vesicles were disrupted and the nanoparticles escaped the vesicles floating freely in the cytoplasm. Flow cytometry showed that all the cells effectively interact with nanoparticles. This interaction leads to cell granularity change. Specifically, A 549, MDCK, and LE С , and cells treated by nanoparticles have the values of size scattered signal 16 ? 2, 20 ? 3, and 39 ? 3%, respectively, com-paring with the untreated cells. The Pr3+:LaF 3 (C Pr = 1%) nanoparticles were not found into the cellular or-ganelles. The cytotoxicity of the Pr3+:LaF 3 (C Pr = 1%) nanoparticles is not significant at concentrations of 0.05,0.1, 0.25, and 0.5 g/L	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Cellular uptake and cytotoxicity of unmodified Pr3+:LaF 3 nanoparticles	ru_RU
dc.type	Articles in international journals and collections	ru_RU