KFU. Card publication. Accelerated and efficient method for isolating microRNA from human blood plasma

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

ACCELERATED AND EFFICIENT METHOD FOR ISOLATING MICRORNA FROM HUMAN BLOOD PLASMA

Form of presentation

Articles in Russian journals and collections

Year of publication

2019

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Authors, employees of KFU

Shakh Makhmud Raikhan , author

Authors, students KFU

Letova Irina Alekseevna, author

Bibliographic description in the original language

Letova I. A. , Madumarov S. A., Sysoyeva M. A., Shah Mahmud R. Z. Accelerated and efficient method for isolating microRNA from human blood plasma / IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA // 2019. V.9, P.53-59

Annotation

IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA

Keywords

microRNA; blood plasma; diagnostics; biomarker

The name of the journal

IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA

URL

http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=4&SID=E1chrQmzRcRrjdIwXS3&page=1&doc=1

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=207141&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Shakh Makhmud Raikhan	ru_RU
dc.contributor.author	Letova Irina Alekseevna	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Letova I. A. , Madumarov S. A., Sysoyeva M. A., Shah Mahmud R. Z. Accelerated and efficient method for isolating microRNA from human blood plasma / IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA // 2019. V.9, P.53-59	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=207141&p_lang=2	ru_RU
dc.description.abstract	IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA	ru_RU
dc.description.abstract	The study is dedicated to solving the problem of microRNA extraction from human blood for further use of the microRNA profile in the diagnosis of various diseases. The aim of the work is to choose an effective, accelerated method for the isolation of microRNA from human blood plasma, sufficient in quantity for diagnosing in clinical practice. Comparison of three methods for obtaining microRNA was carried out: 1) using a silicon oxide-based QIAamp RNA Blood Mini Kit column, 2) phenol-chloroform extraction using TRIzol LS Reagent and 3) phenol-chloroform extraction using TRIzol Reagent. It was established that for the most complete isolation of total human RNA from human blood plasma in order to obtain microRNA, TRIzol LS Reagent can be efficiently used. Because its use allows obtaining microRNA in less time - 30-40 min, and in an amount sufficient for clinical analysis (total RNA - up to 0,116 ng/mu l, and microRNA - 350,3 picograms/mu l), and also with the least amount of concomitant messenger RNA and long non-coding RNA. Using TRIzol LS Reagent allows you to increase the output of miRNA by 2,7 times compared with the use of TRIzol Reagent	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Accelerated and efficient method for isolating microRNA from human blood plasma	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU