KFU. Card publication. Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase

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ELECTROCHEMICAL BIOSENSOR BASED ON POLYELECTROLYTE COMPLEXES FOR THE DETERMINATION OF REVERSIBLE INHIBITORS OF ACETYLCHOLINESTERASE

Form of presentation

Articles in international journals and collections

Year of publication

2019

Authors, employees of KFU

Ivanov Aleksey Nikolaevich, author

Other authors

Sharafieva Indira

Authors, postgraduates KFU

Davletshina Regina Rudisovna, postgraduate kfu

Bibliographic description in the original language

Alexey Ivanov, Regina Davletshina, Indira Sharafieva and Gennady Evtugyn, Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase, Talanta, https://doi.org/10.1016/j.talanta.2018.10.100

Annotation

Reversible inhibitors of acetylcholinesterase are increasingly applied in the Alzheimer's disease therapy. Their determination with biosensors requires special attention to the enzyme immobilization due to importance of electrostatic and steric factors affecting accessibility of the active site to the substrate and inhibitor present simultaneously. In this work, polyelectrolyte complexes have been assembled from electrochemically inactive components with implementation of acetylcholinesterase from electric eel. The influence of the complex components and enzyme position on the assembling efficiency was explored using surface plasmon resonance and kinetic parameters of the enzyme with amperometry with Co phtalocyanine mediator. The biosensor developed showed robust response toward donepezil and berberine with limit of detection of 0.46 and 70 nM, respectively, within 1 minute after inhibitor addition. The sensitivity of determination coincides or is better than characteristics of biosen

Keywords

acetylcholinesterase biosensor,reversible inhibition,polyelectrolyte complex,Alzheimer's disease drug

Place of publication

Netherlands

The name of the journal

TALANTA

URL

https://doi.org/10.1016/j.talanta.2018.10.100

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=187678&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ivanov Aleksey Nikolaevich	ru_RU
dc.contributor.author	Davletshina Regina Rudisovna	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Alexey Ivanov, Regina Davletshina, Indira Sharafieva and Gennady Evtugyn, Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase, Talanta, https://doi.org/10.1016/j.talanta.2018.10.100	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=187678&p_lang=2	ru_RU
dc.description.abstract	TALANTA	ru_RU
dc.description.abstract	Reversible inhibitors of acetylcholinesterase are increasingly applied in the Alzheimer's disease therapy. Their determination with biosensors requires special attention to the enzyme immobilization due to importance of electrostatic and steric factors affecting accessibility of the active site to the substrate and inhibitor present simultaneously. In this work, polyelectrolyte complexes have been assembled from electrochemically inactive components with implementation of acetylcholinesterase from electric eel. The influence of the complex components and enzyme position on the assembling efficiency was explored using surface plasmon resonance and kinetic parameters of the enzyme with amperometry with Co phtalocyanine mediator. The biosensor developed showed robust response toward donepezil and berberine with limit of detection of 0.46 and 70 nM, respectively, within 1 minute after inhibitor addition. The sensitivity of determination coincides or is better than characteristics of biosen	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	acetylcholinesterase biosensor	ru_RU
dc.subject	reversible inhibition	ru_RU
dc.subject	polyelectrolyte complex	ru_RU
dc.subject	Alzheimer's disease drug	ru_RU
dc.title	Electrochemical biosensor based on polyelectrolyte complexes for the determination of reversible inhibitors of acetylcholinesterase	ru_RU
dc.type	Articles in international journals and collections	ru_RU