KFU. Card publication. Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine

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REACTIVATION OF SERRATIA MARCESCENS MUTANT ENDONUCLEASE BY HYDROXILAMINE

Form of presentation

Articles in international journals and collections

Year of publication

2018

Язык

английский

Authors, employees of KFU

Gimadutdinov Oleg Aleksandrovich, author

Trushin Maksim Viktorovich, author

Khamidullina Raisa Gusmanovna, author

Authors, students KFU

Fazleeva Ilmira Ildarovna, author

Bibliographic description in the original language

R.G. Khamidullina. Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine/R.G. Khamidullina, I.I. Fazleeva, M.V.Trushin, O.A. Gimadutdinov///J. Pharm. Sci. & Res. Vol. 10(9), 2018, 2341-2345

Annotation

It is known that histidine plays an important role in the catalytic activity of many nucleases. Performing the function of a common base of these enzymes, it activates the formation of hydroxyl from the water molecule, which, in turn, by attacking the phosphorus atom of the diester bond causes its rupture. It was previously shown that in the endonuclease Serratia marcescens, the replacement of histidine with glycine results in its inactivation. We were able to restore the hydroxylamine activity of the mutant enzyme Serratia marcescens endonuclease, in which histidine in the 89th position is replaced by glycine.

Keywords

Key words: endonuclease, plasmid, basal level of expression, hydroxylamine, histidine.

The name of the journal

Journal of Pharmaceutical Sciences and Research

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=187009&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gimadutdinov Oleg Aleksandrovich	ru_RU
dc.contributor.author	Trushin Maksim Viktorovich	ru_RU
dc.contributor.author	Khamidullina Raisa Gusmanovna	ru_RU
dc.contributor.author	Fazleeva Ilmira Ildarovna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	R.G. Khamidullina. Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine/R.G. Khamidullina, I.I. Fazleeva, M.V.Trushin, O.A. Gimadutdinov///J. Pharm. Sci. & Res. Vol. 10(9), 2018, 2341-2345	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=187009&p_lang=2	ru_RU
dc.description.abstract	Journal of Pharmaceutical Sciences and Research	ru_RU
dc.description.abstract	It is known that histidine plays an important role in the catalytic activity of many nucleases. Performing the function of a common base of these enzymes, it activates the formation of hydroxyl from the water molecule, which, in turn, by attacking the phosphorus atom of the diester bond causes its rupture. It was previously shown that in the endonuclease Serratia marcescens, the replacement of histidine with glycine results in its inactivation. We were able to restore the hydroxylamine activity of the mutant enzyme Serratia marcescens endonuclease, in which histidine in the 89th position is replaced by glycine.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Key words: endonuclease	ru_RU
dc.subject	plasmid	ru_RU
dc.subject	basal level of expression	ru_RU
dc.subject	hydroxylamine	ru_RU
dc.subject	histidine.	ru_RU
dc.title	Reactivation of Serratia marcescens Mutant Endonuclease by Hydroxilamine	ru_RU
dc.type	Articles in international journals and collections	ru_RU