KFU. Card publication. Histochemical analysis of self-organizing 3D spheroid-like cultures of adipose-derived mesenchymal stem cells

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FEDERAL UNIVERSITY

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HISTOCHEMICAL ANALYSIS OF SELF-ORGANIZING 3D SPHEROID-LIKE CULTURES OF ADIPOSE-DERIVED MESENCHYMAL STEM CELLS

Form of presentation

Conference proceedings in international journals and collections

Year of publication

2018

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Authors, employees of KFU

Gumerova Anisa Azatovna, author

Kiyasov Andrey Pavlovich, author

Rizvanov Albert Anatolevich, author

Titova Angelina Andreevna, author

Shafigullina Aygul Kasyymovna, author

Bibliographic description in the original language

Shafigullina A. Histochemical analysis of self-organizing 3D spheroid-like cultures of adipose-derived mesenchymal stem cells / A. Shafigullina, A. Titova, A. Rizvanov, A. Gumerova, A. Kiassov // 52nd Annual Scientific Meeting of ESCI, 29 May -1 June 2018, Barcelona, Spain. - European Journal of Clinical Investigation - May, 2018 - Vol.48 (Suppl.1) - P.91.

Annotation

52nd Annual Scientific Meeting of ESCI, 29 May -1 June 2018, Barcelona, Spain

Keywords

Histochemical analysis, 3D spheroid-like cultures, adipose-derived mesenchymal stem cells, mesenchymal-epithelial transformation, epithelial-mesenchymal transformation

The name of the journal

52nd Annual Scientific Meeting of ESCI, 29 May -1 June 2018, Barcelona, Spain

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/131560/F_tezis_2_ESCI_2018_final_version.pdf?sequence=2&isAllowed=y

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=181997&p_lang=2

Resource files

File name	Size (MB)	Format
F_tezis_2_ESCI_2018_final_version.pdf	0,17	pdf	show / download
F_oblozhka_tezisov_ESCI_2018.jpg	0,02	jpg	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gumerova Anisa Azatovna	ru_RU
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.contributor.author	Shafigullina Aygul Kasyymovna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Shafigullina A. Histochemical analysis of self-organizing 3D spheroid-like cultures of adipose-derived mesenchymal stem cells / A. Shafigullina, A. Titova, A. Rizvanov, A. Gumerova, A. Kiassov // 52nd Annual Scientific Meeting of ESCI, 29 May -1 June 2018, Barcelona, Spain. - European Journal of Clinical Investigation - May, 2018 - Vol.48 (Suppl.1) - P.91.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=181997&p_lang=2	ru_RU
dc.description.abstract	52nd Annual Scientific Meeting of ESCI, 29 May -1 June 2018, Barcelona, Spain	ru_RU
dc.description.abstract	Histochemical staining demonstrated fusion of cells with formation of syncytium-like structures. On the periphery cells were polygonal, round, had epithelial morphology. Groups of cells organized in line inside spheroid remained elongated AD-MSC shape. Most of the cells did not proliferate. There were no migrating α SMA+ cells. Polygonal cells on the periphery and a few cells inside the spheroid were desmin+. Interestingly, peripheral and elongated shape cells expressed epithelial marker CK-19+. Probably, within spheroid there was spontaneous mesenchymal-epithelial transdifferentiation. When spheroids were placed in a new culture dish we observed explantational outgrowth of spindle-shaped AD-MSC suggesting that cells within spheroids were alive, able to outgrow and revert through epithelial-mesenchymal transdifferentiation back to MSC phenotype. Investigation was funded by RFBR grant 18-04-01133 and supported by Program of Competitive Growth of KFU.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Histochemical analysis	ru_RU
dc.subject	3D spheroid-like cultures	ru_RU
dc.subject	adipose-derived mesenchymal stem cells	ru_RU
dc.subject	mesenchymal-epithelial transformation	ru_RU
dc.subject	epithelial-mesenchymal transformation	ru_RU
dc.title	Histochemical analysis of self-organizing 3D spheroid-like cultures of adipose-derived mesenchymal stem cells	ru_RU
dc.type	Conference proceedings in international journals and collections	ru_RU