KFU. Card publication. Insights into the molecular interaction between sucrose and a-chymotrypsin

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
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INSIGHTS INTO THE MOLECULAR INTERACTION BETWEEN SUCROSE AND A-CHYMOTRYPSIN

Form of presentation

Articles in international journals and collections

Year of publication

2018

Язык

английский

Authors, employees of KFU

Sirotkin Vladimir Aleksandrovich, author

Bibliographic description in the original language

Farhadian, S. Insights into the molecular interaction between sucrose and a-chymotrypsin. [Text]/ S. Farhadian, B. Shareghi, L. Momeni, O. K.Abou-Zied, V.A. Sirotkin, M. Tachiya, A.A. Saboury // International Journal of Biological Macromolecules. - 2018. - Vol. 114. - P.950-960.

Annotation

One of the most important purposes of enzyme engineering is to increase the thermal and kinetic stability of enzymes, which is an important factor for using enzymes in industry. The purpose of the present study is to achieve a higher thermal stability of α-chymotrypsin (α-Chy) by modification of the solvent environment. The influence of sucrose was investigated using thermal denaturation analysis, fluorescence spectroscopy, circular dichroism, molecular docking and molecular dynamics (MD) simulations. The results point to the effect of sucrose in enhancing the α-Chy stability. Fluorescence spectroscopy revealed one binding site that is dominated by static quenching. Molecular docking and MD simulation results indicate that hydrogen bonding and van der Waals forces play a major role in stabilizing the complex. Tm of this complex was enhanced due to the higher H-bond formation and the lower surface hydrophobicity after sucrose modification. The results show the ability of sucrose in prot

Keywords

chymotrypsin, sucrose, enzyme engineering, thermal denaturation analysis, fluorescence spectroscopy, circular dichroism, molecular docking, molecular dynamics simulations

The name of the journal

International Journal of Biological Macromolecules

URL

https://www.sciencedirect.com/science/article/pii/S0141813018300990

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=179420&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sirotkin Vladimir Aleksandrovich	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Farhadian, S. Insights into the molecular interaction between sucrose and a-chymotrypsin. [Text]/ S. Farhadian, B. Shareghi, L. Momeni, O. K.Abou-Zied, V.A. Sirotkin, M. Tachiya, A.A. Saboury // International Journal of Biological Macromolecules. - 2018. - Vol. 114. - P.950-960.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=179420&p_lang=2	ru_RU
dc.description.abstract	International Journal of Biological Macromolecules	ru_RU
dc.description.abstract	One of the most important purposes of enzyme engineering is to increase the thermal and kinetic stability of enzymes, which is an important factor for using enzymes in industry. The purpose of the present study is to achieve a higher thermal stability of α-chymotrypsin (α-Chy) by modification of the solvent environment. The influence of sucrose was investigated using thermal denaturation analysis, fluorescence spectroscopy, circular dichroism, molecular docking and molecular dynamics (MD) simulations. The results point to the effect of sucrose in enhancing the α-Chy stability. Fluorescence spectroscopy revealed one binding site that is dominated by static quenching. Molecular docking and MD simulation results indicate that hydrogen bonding and van der Waals forces play a major role in stabilizing the complex. Tm of this complex was enhanced due to the higher H-bond formation and the lower surface hydrophobicity after sucrose modification. The results show the ability of sucrose in prot	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	chymotrypsin	ru_RU
dc.subject	sucrose	ru_RU
dc.subject	enzyme engineering	ru_RU
dc.subject	thermal denaturation analysis	ru_RU
dc.subject	fluorescence spectroscopy	ru_RU
dc.subject	circular dichroism	ru_RU
dc.subject	molecular docking	ru_RU
dc.subject	molecular dynamics simulations	ru_RU
dc.title	Insights into the molecular interaction between sucrose and a-chymotrypsin	ru_RU
dc.type	Articles in international journals and collections	ru_RU