KFU. Card publication. Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images

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FAST AND SIMPLE TOOL FOR THE QUANTIFICATION OF BIOFILM-EMBEDDED CELLS SUB-POPULATIONS FROM FLUORESCENT MICROSCOPIC IMAGES

Form of presentation

Articles in international journals and collections

Year of publication

2017

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Authors, employees of KFU

Baydamshina Diana Rafisovna, author

Bogachev Mikhail Igorevich, author

Zelenikhin Pavel Valerevich, author

Kayumov Ayrat Rashitovich, author

Murtazina Regina Raufovna, author

Trizna Elena Yurevna, author

Other authors

Markelov O. , author

Bibliographic description in the original language

Bogachev M. Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images / M.Bogachev, O. Markelov, E. Trizna, D. Baydamshina, P. Zelenikhin, R. Murtazina, A. Kayumov // BioRxiv. - 2017. – P.181495.

Annotation

Fluorescent staining is a common tool for both quantitative and qualitative assessment of pro- and eukaryotic cells sub-population fractions by using microscopy and flow cytometry. However, direct cell counting by flow cytometry is often limited, for example when working with cells rigidly adhered either to each other or to external surfaces like in bacterial biofilms or adherent cell lines and tissue samples. An alternative approach is provided by using fluorescent microscopy and confocal laser scanning microscopy (CLSM), which enables the evaluation of fractions of cells subpopulations in a given sample. To facilitate the quantitative assessment of cell fractions in microphotographs, we suggest a simple two-step algorithm that combines the cell selection based and the statistical approaches. Based on a series of experimental measurements performed on bacterial and eukaryotic cells under various measurement conditions, we show explicitly that the suggested approach effectively account

Keywords

Biofilm

The name of the journal

BioRxiv

URL

https://www.biorxiv.org/content/early/2017/08/29/181495

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=174577&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Baydamshina Diana Rafisovna	ru_RU
dc.contributor.author	Bogachev Mikhail Igorevich	ru_RU
dc.contributor.author	Zelenikhin Pavel Valerevich	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Murtazina Regina Raufovna	ru_RU
dc.contributor.author	Trizna Elena Yurevna	ru_RU
dc.contributor.author	Markelov O.	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Bogachev M. Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images / M.Bogachev, O. Markelov, E. Trizna, D. Baydamshina, P. Zelenikhin, R. Murtazina, A. Kayumov // BioRxiv. - 2017. – P.181495.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=174577&p_lang=2	ru_RU
dc.description.abstract	BioRxiv	ru_RU
dc.description.abstract	Fluorescent staining is a common tool for both quantitative and qualitative assessment of pro- and eukaryotic cells sub-population fractions by using microscopy and flow cytometry. However, direct cell counting by flow cytometry is often limited, for example when working with cells rigidly adhered either to each other or to external surfaces like in bacterial biofilms or adherent cell lines and tissue samples. An alternative approach is provided by using fluorescent microscopy and confocal laser scanning microscopy (CLSM), which enables the evaluation of fractions of cells subpopulations in a given sample. To facilitate the quantitative assessment of cell fractions in microphotographs, we suggest a simple two-step algorithm that combines the cell selection based and the statistical approaches. Based on a series of experimental measurements performed on bacterial and eukaryotic cells under various measurement conditions, we show explicitly that the suggested approach effectively account	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images	ru_RU
dc.type	Articles in international journals and collections	ru_RU