KFU. Card publication. Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches

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SIMPLE GD3+-NEU5NAC COMPLEXATION RESULTS IN NMR CHEMICAL SHIFT ASYMMETRIES OF STRUCTURALLY EQUIVALENT COMPLEX-TYPE N-GLYCAN BRANCHES

Form of presentation

Articles in international journals and collections

Year of publication

2017

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Authors, employees of KFU

Garaeva Nataliya Sergeevna, author

Klochkov Vladimir Vasilevich, author

Kurbangalieva Almira Rafaelovna, author

Tanaka Kacunori , author

Usachev Konstantin Sergeevich, author

Bibliographic description in the original language

Usachev K. Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches / K. Usachev, Y. Yamaguchi, M. Takamatsu, N. Pavlova, V. Klochkov, A. Kurbangalieva, T. Murase, T. Shimoda, K. Tanaka // Analyst. – 2017. – V. 142. – P. 2897–2900.

Annotation

In the present Communication, we propose a quite simple but previously overlooked approach for conveniently analyzing, assigning, and extracting sialic acid-containing N-glycan structures using high-resolution NMR spectroscopy without pre-installing metal chelators. Paramagnetic metals, such as Gd3+, appear to bind to the carboxyl groups of N-acetylneuraminic acid when introduced at room temperature, leading to the measurement of nonequivalent proton and carbon NMR spectral signals among otherwise “identical” glycan branched structures.

Keywords

N-glycan, sialic acid, NMR spectroscopy, paramagnetic metal

The name of the journal

Analyst

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027044188&doi=10.1039%2fc7an00817a&partnerID=40&md5=2d30761e615ba9ae83363e1b8c60624a

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=163547&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Garaeva Nataliya Sergeevna	ru_RU
dc.contributor.author	Klochkov Vladimir Vasilevich	ru_RU
dc.contributor.author	Klochkov Vladimir Vasilevich	ru_RU
dc.contributor.author	Kurbangalieva Almira Rafaelovna	ru_RU
dc.contributor.author	Tanaka Kacunori	ru_RU
dc.contributor.author	Usachev Konstantin Sergeevich	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Usachev K. Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches / K. Usachev, Y. Yamaguchi, M. Takamatsu, N. Pavlova, V. Klochkov, A. Kurbangalieva, T. Murase, T. Shimoda, K. Tanaka // Analyst. – 2017. – V. 142. – P. 2897–2900.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=163547&p_lang=2	ru_RU
dc.description.abstract	Analyst	ru_RU
dc.description.abstract	In the present Communication, we propose a quite simple but previously overlooked approach for conveniently analyzing, assigning, and extracting sialic acid-containing N-glycan structures using high-resolution NMR spectroscopy without pre-installing metal chelators. Paramagnetic metals, such as Gd3+, appear to bind to the carboxyl groups of N-acetylneuraminic acid when introduced at room temperature, leading to the measurement of nonequivalent proton and carbon NMR spectral signals among otherwise “identical” glycan branched structures.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	N-glycan	ru_RU
dc.subject	sialic acid	ru_RU
dc.subject	NMR spectroscopy	ru_RU
dc.subject	paramagnetic metal	ru_RU
dc.title	Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches	ru_RU
dc.type	Articles in international journals and collections	ru_RU