KFU. Card publication. Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene

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SPECIES-SPECIFIC DIFFERENCES IN PEROXISOME PROLIFERATION, CATALASE, AND SOD2 UPREGULATION AS WELL AS TOXICITY IN HUMAN, MOUSE, AND RAT HEPATOMA CELLS INDUCED BY THE EXPLOSIVE AND ENVIRONMENTAL POLLUTANT 2,4,6-TRINITROTOLUENE

Form of presentation

Articles in international journals and collections

Year of publication

2017

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Authors, employees of KFU

Naumenko Ekaterina Anatolevna, author

Bibliographic description in the original language

Naumenko, E.A. Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene / E.A. Naumenko , B. Ahlemeyer, E. Baumgart-Vogt // Environ Toxicol. – 2017. – V. 32(3) – P. 989-1006

Annotation

2,4,6-Trinitrotoluene (TNT) has been widely used as an explosive substance and its toxicity is still of interest as it persisted in polluted areas. TNT is metabolized in hepatocytes which are prone to its toxicity. Since analysis of the human liver or hepatocytes is restricted due to ethical reasons, we investigated the effects of TNT on cell viability, reactive oxygen species (ROS) production, peroxisome proliferation, and antioxidative enzymes in human (HepG2), mouse (Hepa 1-6), and rat (H4IIEC3) hepatoma cell lines. Under control conditions, hepatoma cells of all three species were highly comparable exhibiting identical proliferation rates and distribution of their cell cycle phases. However, we found strong differences in TNT toxicity with the lowest IC50 values (highest cell death rate) for rat cells, whereas human and mouse cells were three to sevenfold less sensitive. Moreover, a strong decrease in cellular dehydrogenase activity (MTT assay) and increased ROS levels were noted.

Keywords

Species-specific differences, peroxisome proliferation, 2,4,6-trinitrotoluene

The name of the journal

Environmental Toxicology

URL

http://onlinelibrary.wiley.com/doi/10.1002/tox.22299/abstract;jsessionid=0C4AB62C762812F187CE16E65C5BD21E.f03t04

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=159814&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Naumenko Ekaterina Anatolevna	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Naumenko, E.A. Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene / E.A. Naumenko , B. Ahlemeyer, E. Baumgart-Vogt // Environ Toxicol. – 2017. – V. 32(3) – P. 989-1006	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=159814&p_lang=2	ru_RU
dc.description.abstract	Environmental Toxicology	ru_RU
dc.description.abstract	2,4,6-Trinitrotoluene (TNT) has been widely used as an explosive substance and its toxicity is still of interest as it persisted in polluted areas. TNT is metabolized in hepatocytes which are prone to its toxicity. Since analysis of the human liver or hepatocytes is restricted due to ethical reasons, we investigated the effects of TNT on cell viability, reactive oxygen species (ROS) production, peroxisome proliferation, and antioxidative enzymes in human (HepG2), mouse (Hepa 1-6), and rat (H4IIEC3) hepatoma cell lines. Under control conditions, hepatoma cells of all three species were highly comparable exhibiting identical proliferation rates and distribution of their cell cycle phases. However, we found strong differences in TNT toxicity with the lowest IC50 values (highest cell death rate) for rat cells, whereas human and mouse cells were three to sevenfold less sensitive. Moreover, a strong decrease in cellular dehydrogenase activity (MTT assay) and increased ROS levels were noted.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Species-specific differences	ru_RU
dc.subject	peroxisome proliferation	ru_RU
dc.subject	2	ru_RU
dc.subject	4	ru_RU
dc.subject	6-trinitrotoluene	ru_RU
dc.title	Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene	ru_RU
dc.type	Articles in international journals and collections	ru_RU