KFU. Card publication. Preferential Solvation/Hydration of α-Chymotrypsin in Water

KAZAN
FEDERAL UNIVERSITY

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PREFERENTIAL SOLVATION/HYDRATION OF α-CHYMOTRYPSIN IN WATER&NDASH;ACETONITRILE MIXTURES

Form of presentation

Articles in international journals and collections

Year of publication

2017

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Authors, employees of KFU

Sirotkin Vladimir Aleksandrovich, author

Authors, students KFU

Kuchierskaya Aleksandra Aleksandrovna, author

Bibliographic description in the original language

Sirotkin, V.A. Preferential Solvation/Hydration of α-Chymotrypsin in Water–Acetonitrile Mixtures [Text]/ V. A. Sirotkin, A.A. Kuchierskaya // Journal of Physical Chemistry B. - 2017. - Vol. 121. - P. 4422-4430. ISSN: 1520-6106.

Annotation

The aim of our study is to monitor the preferential hydration/solvation of the protein macromolecules at low and high water content in water–organic mixtures. Our approach is based on the analysis of the absolute values of the water/organic solvent sorption. We applied this approach to estimate the protein stabilization/destabilization due to the preferential interactions of α-chymotrypsin with water–acetonitrile mixtures. Advantages of our approach: (i) The preferential interaction parameters can be determined in the entire range of water content in water–organic mixtures. (ii) Our approach facilitates the individual evaluation of the Gibbs energies of water, protein, and organic solvent.

Keywords

Preferential solvation, Protein hydration, Water, Organic solvent, Acetonitrile

The name of the journal

J PHYS CHEM B

URL

http://pubs.acs.org/doi/abs/10.1021/acs.jpcb.7b01632

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=157387&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sirotkin Vladimir Aleksandrovich	ru_RU
dc.contributor.author	Kuchierskaya Aleksandra Aleksandrovna	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Sirotkin, V.A. Preferential Solvation/Hydration of α-Chymotrypsin in Water–Acetonitrile Mixtures [Text]/ V. A. Sirotkin, A.A. Kuchierskaya // Journal of Physical Chemistry B. - 2017. - Vol. 121. - P. 4422-4430. ISSN: 1520-6106.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=157387&p_lang=2	ru_RU
dc.description.abstract	J PHYS CHEM B	ru_RU
dc.description.abstract	The aim of our study is to monitor the preferential hydration/solvation of the protein macromolecules at low and high water content in water–organic mixtures. Our approach is based on the analysis of the absolute values of the water/organic solvent sorption. We applied this approach to estimate the protein stabilization/destabilization due to the preferential interactions of α-chymotrypsin with water–acetonitrile mixtures. Advantages of our approach: (i) The preferential interaction parameters can be determined in the entire range of water content in water–organic mixtures. (ii) Our approach facilitates the individual evaluation of the Gibbs energies of water, protein, and organic solvent.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Preferential solvation	ru_RU
dc.subject	Protein hydration	ru_RU
dc.subject	Water	ru_RU
dc.subject	Organic solvent	ru_RU
dc.subject	Acetonitrile	ru_RU
dc.title	Preferential Solvation/Hydration of α-Chymotrypsin in Water–Acetonitrile Mixtures	ru_RU
dc.type	Articles in international journals and collections	ru_RU