KFU. Card publication. Exogenous Bacillus pumilus RNase (binase) suppresses the reproduction of reovirus serotype 1

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FEDERAL UNIVERSITY

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EXOGENOUS BACILLUS PUMILUS RNASE (BINASE) SUPPRESSES THE REPRODUCTION OF REOVIRUS SEROTYPE 1

Form of presentation

Articles in international journals and collections

Year of publication

2017

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Authors, employees of KFU

Zelenikhin Pavel Valerevich, author

Ilinskaya Olga Nikolaevna, author

Kolpakov Aleksey Ivanovich, author

Shakh Makhmud Raikhan , author

Other authors

Efimova Marina Anatolevna, author

Authors, students KFU

Sabirova Milyausha Ilsurovna, author

Bibliographic description in the original language

Efimova M. A. Exogenous Bacillus pumilus RNase (binase) suppresses the reproduction of reovirus serotype 1 / M. A. Efimova, R. Shah Mahmud, P. V. Zelenikhin, M. I. Sabirova, A. I. Kolpakov, O. N. Ilinskaya // Molecular Biology. - 2017. - V. 51, I. 1. - P. 96–101.

Annotation

The experimental study identified the antiviral activity of Bacillus pumilus RNase (binase) against the reovirus of serotype 1/strain Lang. For the first time, it has been found that 50 μg/mL of binase effectively reduced the hemagglutinin and cytocidal activity of reovirus in Vero cell line. The preincubation of the enzyme with reovirus before infection of the cells inhibited the viral replication. To determine the stagedependent effect of reovirus reproduction upon binase inhibition, the infected cells were treated with binase or RNase A at different phases of the infectious cycle. The treatment of virus-infected cells has revealed that both enzymes have a maximal antiviral effect on the reovirus propagation during early phases of the reovirus reproduction cycle, with binase being more effective than RNase A. It has been hypothesized that the combined action of the oncolytic reovirus and binase is promising for the elimination of tumor cells carrying mutated RAS gene.

Keywords

binase, Bacillus pumilus, RNase, reovirus, antiviral agent

The name of the journal

Molecular Biology

URL

http://rd.springer.com/article/10.1134/S0026893316060042

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=152171&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Zelenikhin Pavel Valerevich	ru_RU
dc.contributor.author	Ilinskaya Olga Nikolaevna	ru_RU
dc.contributor.author	Kolpakov Aleksey Ivanovich	ru_RU
dc.contributor.author	Shakh Makhmud Raikhan	ru_RU
dc.contributor.author	Efimova Marina Anatolevna	ru_RU
dc.contributor.author	Sabirova Milyausha Ilsurovna	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Efimova M. A. Exogenous Bacillus pumilus RNase (binase) suppresses the reproduction of reovirus serotype 1 / M. A. Efimova, R. Shah Mahmud, P. V. Zelenikhin, M. I. Sabirova, A. I. Kolpakov, O. N. Ilinskaya // Molecular Biology. - 2017. - V. 51, I. 1. - P. 96–101.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=152171&p_lang=2	ru_RU
dc.description.abstract	Molecular Biology	ru_RU
dc.description.abstract	The experimental study identified the antiviral activity of Bacillus pumilus RNase (binase) against the reovirus of serotype 1/strain Lang. For the first time, it has been found that 50 μg/mL of binase effectively reduced the hemagglutinin and cytocidal activity of reovirus in Vero cell line. The preincubation of the enzyme with reovirus before infection of the cells inhibited the viral replication. To determine the stagedependent effect of reovirus reproduction upon binase inhibition, the infected cells were treated with binase or RNase A at different phases of the infectious cycle. The treatment of virus-infected cells has revealed that both enzymes have a maximal antiviral effect on the reovirus propagation during early phases of the reovirus reproduction cycle, with binase being more effective than RNase A. It has been hypothesized that the combined action of the oncolytic reovirus and binase is promising for the elimination of tumor cells carrying mutated RAS gene.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	binase	ru_RU
dc.subject	Bacillus pumilus	ru_RU
dc.subject	RNase	ru_RU
dc.subject	reovirus	ru_RU
dc.subject	antiviral agent	ru_RU
dc.title	Exogenous Bacillus pumilus RNase (binase) suppresses the reproduction of reovirus serotype 1	ru_RU
dc.type	Articles in international journals and collections	ru_RU