KFU. Card publication. Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype

KAZAN
FEDERAL UNIVERSITY

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EFFECT OF ADENOVIRAL TRANSDUCTION OF HEPATIC STELLATE CELLS WITH ADV5-OPTHGF-RFP ON THEIR PHENOTYPE

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Garanina Ekaterina Evgenevna, author

Gumerova Anisa Azatovna, author

Zaikina Elvira Ildarovna, author

Kiyasov Andrey Pavlovich, author

Mavlikeev Mikhail Olegovich, author

Rizvanov Albert Anatolevich, author

Sakhapov Damir Ilgizovich, author

Titova Angelina Andreevna, author

Shafigullina Aygul Kasyymovna, author

Authors, postgraduates KFU

Mavlikeev Mikhail Olegovich, postgraduate kfu

Titova Angelina Andreevna, postgraduate kfu

Bibliographic description in the original language

Shafigullina, A. K. Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype / A.K. Shafigullina, E.I. Zaikina, E.E. Garanina, D.I. Sakhapov, A.A. Titova, M.O. Mavlikeev, A.A. Rizvanov, A.A. Gumerova, A.A. Kiassov // BioNanoScience. - 2016. - Vol. 6. - R. 1-4.

Annotation

Adenovirus is a promising gene delivery vector that has a high efficiency and relative ease of construction. These advantages make this system attractive for diverse research applications. In this project we performed transduction of hepatics stellate cells (HSC) with adenoviral vector containing hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene didn`t change their morphology, but stimulated expression of HSC marker desmin, lead to HSC activation (α-SMA) and appearance of α-FP, one of the hepatoblasts markers. During the entire ex

Keywords

hepatic stellate cells, adenovirus, transduction, differentiation

The name of the journal

BioNanoScience

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/108481/Bionanoscience_Aygul_Phenotype_of_HSC.pdf?sequence=1&isAllowed=y

URL

http://link.springer.com/article/10.1007/s12668-016-0378-7

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=147227&p_lang=2

Resource files

File name	Size (MB)	Format
Bionanoscience_Aygul_Phenotype_of_HSC.pdf	0,22	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Garanina Ekaterina Evgenevna	ru_RU
dc.contributor.author	Gumerova Anisa Azatovna	ru_RU
dc.contributor.author	Zaikina Elvira Ildarovna	ru_RU
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Mavlikeev Mikhail Olegovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Sakhapov Damir Ilgizovich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.contributor.author	Shafigullina Aygul Kasyymovna	ru_RU
dc.contributor.author	Mavlikeev Mikhail Olegovich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Shafigullina, A. K. Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype / A.K. Shafigullina, E.I. Zaikina, E.E. Garanina, D.I. Sakhapov, A.A. Titova, M.O. Mavlikeev, A.A. Rizvanov, A.A. Gumerova, A.A. Kiassov // BioNanoScience. - 2016. - Vol. 6. - �. 1-4.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=147227&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Adenovirus is a promising gene delivery vector that has a high efficiency and relative ease of construction. These advantages make this system attractive for diverse research applications. In this project we performed transduction of hepatics stellate cells (HSC) with adenoviral vector containing hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene didn`t change their morphology, but stimulated expression of HSC marker desmin, lead to HSC activation (α-SMA) and appearance of α-FP, one of the hepatoblasts markers. During the entire ex	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	hepatic stellate cells	ru_RU
dc.subject	adenovirus	ru_RU
dc.subject	transduction	ru_RU
dc.subject	differentiation	ru_RU
dc.title	Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype	ru_RU
dc.type	Articles in international journals and collections	ru_RU