KFU. Card publication. Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease

KAZAN
FEDERAL UNIVERSITY

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GRANULE ASSOCIATED DNASE IN T-LYMPHOCYTES FROM PATIENTS WITH INFLAMMATORY DISEASE

Form of presentation

Articles in Russian journals and collections

Year of publication

2016

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Authors, employees of KFU

Abramova Zinaida Ivanovna, author

Reshetnikova Irina Dmitrievna, author

Skripova Vera Sergeevna, author

Other authors

Vodounon Cyrille A -, author

Bibliographic description in the original language

Skripov V. Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease /V. Skripov, C.A. Vodoounon, I.D. Reshetnicova, B.B. Legba, Z.I. Abramova // American Journal of Biomedical Research. -2016.- V.4,N.4.-P. 87-93 DOI: 10.12691/ajbr-4-4-2

Annotation

Recent data highlight the undeniable role of programmed cell death type I of lymphocytes in the pathogenesis of certain allergic diseases and autoimmune diseases such as Bronchial Asthma and hemorrhagic rectocolitis. But little data exist on the enzymatic activity of secretory granules associated with lymphocytes of patients suffering from these inflammatory diseases. The aim of the study was to characterize the activity of the DNase in the secretory granules of T lymphocytes isolated from peripheral blood of patients with Bronchial Asthma (n = 20) and Hemorrhagic Rectocolitis (n = 20). Thus, the secretory granules were isolated from lymphocytes by the ultracentrifugation method on percoll density gradient. The detection of the activity of the protein extracts was performed by zymography and electrophoresis method. The results reveal the presence of a protein extract with a molecular weight of 66 kDa both at the level of the granules of the lymphocytes of patients suffering from hemorrhagic rectocolitis considered as a classical autoimmune disease and in the granules of lymphocytes of patients with Bronchial Asthma. The study of physicochemical properties showed an increase in the enzymatic activity of DNase of secretory granules when 1 mM Ca2+ was added to the incubation medium at a pH = 7.5. On the other hand, the addition of 1 mM Zn2 + causes the inhibition of enzyme activity. These results suggest that the enzymatic activity of DNase detected in the granule of lymphocytes of patients with Bronchial Asthma and hemorrhagic rectocolitis occurs not only in the fragmentation of double stranded DNA but could also play a role in the apoptotic process of these same T-lymphocytes. The study of the properties of this DNase in the inflammatory diseases could enable to use this protein as a marker for determining the severity of disease.

Keywords

DNase, secretory granules, Bronchial Asthma, Hemorrhagic Rectocolitis

The name of the journal

American Journal of Biomedical Research

URL

http://pubs.sciepub.com/ajbr/4/4/2/index.html

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=145789&p_lang=2

Resource files

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Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.contributor.author	Reshetnikova Irina Dmitrievna	ru_RU
dc.contributor.author	Skripova Vera Sergeevna	ru_RU
dc.contributor.author	Vodounon Cyrille A -	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Skripov V. Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease /V. Skripov, C.A. Vodoounon, I.D. Reshetnicova, B.B. Legba, Z.I. Abramova // American Journal of Biomedical Research. -2016.- V.4,N.4.-P. 87-93 DOI: 10.12691/ajbr-4-4-2	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=145789&p_lang=2	ru_RU
dc.description.abstract	American Journal of Biomedical Research	ru_RU
dc.description.abstract	Recent data highlight the undeniable role of programmed cell death type I of lymphocytes in the pathogenesis of certain allergic diseases and autoimmune diseases such as Bronchial Asthma and hemorrhagic rectocolitis. But little data exist on the enzymatic activity of secretory granules associated with lymphocytes of patients suffering from these inflammatory diseases. The aim of the study was to characterize the activity of the DNase in the secretory granules of T lymphocytes isolated from peripheral blood of patients with Bronchial Asthma (n = 20) and Hemorrhagic Rectocolitis (n = 20). Thus, the secretory granules were isolated from lymphocytes by the ultracentrifugation method on percoll density gradient. The detection of the activity of the protein extracts was performed by zymography and electrophoresis method. The results reveal the presence of a protein extract with a molecular weight of 66 kDa both at the level of the granules of the lymphocytes of patients suffering from hemorrhagic rectocolitis considered as a classical autoimmune disease and in the granules of lymphocytes of patients with Bronchial Asthma. The study of physicochemical properties showed an increase in the enzymatic activity of DNase of secretory granules when 1 mM Ca2+ was added to the incubation medium at a pH = 7.5. On the other hand, the addition of 1 mM Zn2 + causes the inhibition of enzyme activity. These results suggest that the enzymatic activity of DNase detected in the granule of lymphocytes of patients with Bronchial Asthma and hemorrhagic rectocolitis occurs not only in the fragmentation of double stranded DNA but could also play a role in the apoptotic process of these same T-lymphocytes. The study of the properties of this DNase in the inflammatory diseases could enable to use this protein as a marker for determining the severity of disease.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	DNase	ru_RU
dc.subject	secretory granules	ru_RU
dc.subject	Bronchial Asthma	ru_RU
dc.subject	Hemorrhagic Rectocolitis	ru_RU
dc.title	Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU