KFU. Card publication. EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

EPR DETECTION OF DNA INTERACTION WITH 3-CARBOXY-PROXYL-LABELLED RECOMBINANT HUMAN HISTONE H1.3

Form of presentation

Articles in international journals and collections

Year of publication

2016

��

��

Authors, employees of KFU

Abdullin Timur Ildarovich, author

Gafurov Marat Revgerovich, author

Kamalov Marat Ilyasovich, author

Rizvanov Albert Anatolevich, author

Chasov Vitaliy Vasilevich, author

Bibliographic description in the original language

Chasov V.V. EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3 / V. Chasov, A. Rodionov, M. Gafurov, M. Kamalov,T. Abdullin, S. Zaitsev, A. Rizvanov // BioNanoSci. (2016). DOI: 10.1007/s12668-016-0294-x

Annotation

Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy.

Keywords

Nitroxide spin label, Electron paramagnetic resonance, Recombinant human histone, H1.3, Plasmid DNA, Biosensing

The name of the journal

BioNanoScience

URL

https://doi:10.1007/s12668-016-0294-x

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=145712&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abdullin Timur Ildarovich	ru_RU
dc.contributor.author	Gafurov Marat Revgerovich	ru_RU
dc.contributor.author	Kamalov Marat Ilyasovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Chasov Vitaliy Vasilevich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Chasov V.V. EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3 / V. Chasov, A. Rodionov, M. Gafurov, M. Kamalov,T. Abdullin, S. Zaitsev, A. Rizvanov // BioNanoSci. (2016). DOI: 10.1007/s12668-016-0294-x	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=145712&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Nitroxide spin label	ru_RU
dc.subject	Electron paramagnetic resonance	ru_RU
dc.subject	Recombinant human histone	ru_RU
dc.subject	H1.3	ru_RU
dc.subject	Plasmid DNA	ru_RU
dc.subject	Biosensing	ru_RU
dc.title	EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3	ru_RU
dc.type	Articles in international journals and collections	ru_RU