KFU. Card publication. Surgical Procedure for Extracting Pig Teeth for Isolation and Cultivation of Mesenchymal Stem Cells from Dental Pulp for Regenerative Therapy Applications

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SURGICAL PROCEDURE FOR EXTRACTING PIG TEETH FOR ISOLATION AND CULTIVATION OF MESENCHYMAL STEM CELLS FROM DENTAL PULP FOR REGENERATIVE THERAPY APPLICATIONS

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Masgutov Ruslan Faridovich, author

Masgutova Galina Andreevna, author

Mukhamedshina Yana Olegovna, author

Rizvanov Albert Anatolevich, author

Bibliographic description in the original language

Masgutova G. Surgical Procedure for Extracting Pig Teeth for Isolation and Cultivation of Mesenchymal Stem Cells from Dental Pulp for Regenerative Therapy Applications / G. Masgutova, Y. Mukhamedshina, M. Sergeev, I. Shulman, S. Ogurtsov, R. Masgutov, A. Rizvanov // BioNanoSci. DOI 10.1007/s12668-016-0297-7

Annotation

Our study shows that primary pulp cultures of DP-MSCs are established 5 days after enzymatic diges- tion and 7–10 days following attachment of the digested minced tissue to the bottom of a plate. Though in the first few days the rate of primary ex- pansion for cultures generated by direct isolation was lower than the rate of enzymatic digestion, this differ- ence leveled off on days 14–18 of culture. For DP- MSCs isolation, we recommend the use of deciduous succedaneous lateral incisors and canine teeth of pigs as well as deciduous premolars from the first dentition of young pigs (up to 3 months).

Keywords

Pig teeth extraction, Dental pulp, Mesenchymal stem cells

The name of the journal

BioNanoScience

URL

http://www.readcube.com/articles/10.1007/s12668-016-0297-7?author_access_token=0ouVZCeSJXWVGVgWyMXt3_e4RwlQNchNByi7wbcMAY76fe0e26mPaih3qag_hAdlO2GBJjHOTfZXTjjlWT2PBYi9Io6vczU5ym9zjqlSGCUyhDq7hzGnaOQ3P0JmIbk0pg37CPm56vyeZjRPCrHC4A%3D%3D

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=140751&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Masgutov Ruslan Faridovich	ru_RU
dc.contributor.author	Masgutova Galina Andreevna	ru_RU
dc.contributor.author	Mukhamedshina Yana Olegovna	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Masgutova G. Surgical Procedure for Extracting Pig Teeth for Isolation and Cultivation of Mesenchymal Stem Cells from Dental Pulp for Regenerative Therapy Applications / G. Masgutova, Y. Mukhamedshina, M. Sergeev, I. Shulman, S. Ogurtsov, R. Masgutov, A. Rizvanov // BioNanoSci. DOI 10.1007/s12668-016-0297-7	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=140751&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Our study shows that primary pulp cultures of DP-MSCs are established 5 days after enzymatic diges- tion and 7–10 days following attachment of the digested minced tissue to the bottom of a plate. Though in the first few days the rate of primary ex- pansion for cultures generated by direct isolation was lower than the rate of enzymatic digestion, this differ- ence leveled off on days 14–18 of culture. For DP- MSCs isolation, we recommend the use of deciduous succedaneous lateral incisors and canine teeth of pigs as well as deciduous premolars from the first dentition of young pigs (up to 3 months).	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Pig teeth extraction	ru_RU
dc.subject	Dental pulp	ru_RU
dc.subject	Mesenchymal stem cells	ru_RU
dc.title	Surgical Procedure for Extracting Pig Teeth for Isolation and Cultivation of Mesenchymal Stem Cells from Dental Pulp for Regenerative Therapy Applications	ru_RU
dc.type	Articles in international journals and collections	ru_RU