KFU. Card publication. Propoxylation of cationic polymers provides a novel approach to controllable modulation of their cellular toxicity and interaction with nucleic acids

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PROPOXYLATION OF CATIONIC POLYMERS PROVIDES A NOVEL APPROACH TO CONTROLLABLE MODULATION OF THEIR CELLULAR TOXICITY AND INTERACTION WITH NUCLEIC ACIDS

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Abdullin Timur Ildarovich, author

Badeev Yuriy Vladimirovich, author

Salakhieva Diana Vitalevna, author

Shevchenko Vesta Denisovna, author

Shtyrlin Yuriy Grigorevich, author

Bibliographic description in the original language

Propoxylation of cationic polymers provides a novel approach to controllable modulation of their cellular toxicity and interaction with nucleic acids. Vesta D. Shevchenko, Diana V. Salakhieva, Abdulla A. Yergeshov, Yuriy V. Badeev, Yurii G. Shtyrlin, Timur I. Abdullin //Materials Science and Engineering C 69 (2016) 60–67.

Annotation

An effective chemical approach to modulation of biological interactions of cationic polymers was proposed and tested using polyethyleneimine (PEI) as a drug carrier. Branched 25 kDa PEI was modified in the reaction with propylene oxide (PO) to produce a series of propoxylated PEIs with NH groups grafted by single or oligomer PO units. Clear relationships between the propoxylation degree and biological effects, such as interaction with plasmid DNA, hemolytic, cytotoxic, and pro-apoptotic activities were revealed for PEIs modified upon PO / NH molar ratio of 0.5, 0.75, 1.0 and 3.0. The partial modification of available cationic centers up to 100 % is predominantly accompanied by a significant gradual reduction in polycation adverse effects, while ability of complex formation with plasmid DNA is being preserved. Grafted PEI with 0.75 PO / NH ratio provides better protection from nuclease degradation and transfection activity compared with other modified PEIs.

Keywords

Cationic polymers; Polyethyleneimine; Propoxylation; Biocompatibility; Cellular toxicity; Gene delivery

The name of the journal

Materials Science & Engineering C

URL

http://dx.doi.org/10.1016/j.msec.2016.05.024

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=139057&p_lang=2

Resource files

File name	Size (MB)	Format
_MSEC_PEIs_PO_2016_article.pdf	0,96	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abdullin Timur Ildarovich	ru_RU
dc.contributor.author	Badeev Yuriy Vladimirovich	ru_RU
dc.contributor.author	Salakhieva Diana Vitalevna	ru_RU
dc.contributor.author	Shevchenko Vesta Denisovna	ru_RU
dc.contributor.author	Shtyrlin Yuriy Grigorevich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Propoxylation of cationic polymers provides a novel approach to controllable modulation of their cellular toxicity and interaction with nucleic acids. Vesta D. Shevchenko, Diana V. Salakhieva, Abdulla A. Yergeshov, Yuriy V. Badeev, Yurii G. Shtyrlin, Timur I. Abdullin //Materials Science and Engineering C 69 (2016) 60–67.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=139057&p_lang=2	ru_RU
dc.description.abstract	Materials Science & Engineering C	ru_RU
dc.description.abstract	An effective chemical approach to modulation of biological interactions of cationic polymers was proposed and tested using polyethyleneimine (PEI) as a drug carrier. Branched 25 kDa PEI was modified in the reaction with propylene oxide (PO) to produce a series of propoxylated PEIs with NH groups grafted by single or oligomer PO units. Clear relationships between the propoxylation degree and biological effects, such as interaction with plasmid DNA, hemolytic, cytotoxic, and pro-apoptotic activities were revealed for PEIs modified upon PO / NH molar ratio of 0.5, 0.75, 1.0 and 3.0. The partial modification of available cationic centers up to 100 % is predominantly accompanied by a significant gradual reduction in polycation adverse effects, while ability of complex formation with plasmid DNA is being preserved. Grafted PEI with 0.75 PO / NH ratio provides better protection from nuclease degradation and transfection activity compared with other modified PEIs.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Propoxylation of cationic polymers provides a novel approach to controllable modulation of their cellular toxicity and interaction with nucleic acids	ru_RU
dc.type	Articles in international journals and collections	ru_RU