KFU. Card publication. HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuriesa

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FEDERAL UNIVERSITY

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HTRA PROTEASE FROM BACILLUS SUBTILIS SUPPRESSES THE BACTERIAL FOULING OF THE RAT SKIN INJURIESA

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Akhmetov Nafis Fanisovich, author

Kayumov Ayrat Rashitovich, author

Other authors

Bogachev Mikhail Igorevich, author

Authors, postgraduates KFU

Baltin Maksim Eduardovich, postgraduate kfu

Sharafutdinov Irshad Sultanovich, postgraduate kfu

Authors, students KFU

Shigapova Zukhra Rashidovna, author

Bibliographic description in the original language

Sharafutdinov I. HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuries / I. Sharafutdinov, Z. Shigapova, M. Baltin, N. Akhmetov, M. Bogachev, A. Kayumov // BioNanoScience. – 2016 – V.6(4). – P.564-567.

Annotation

The gene of serine protease HtrA from Bacillus subtilis was cloned and recombinant protein was overexpressed in E.coli and purified. The recombinant HtrA efficiently repressed in vitro the biofilm formation by clinical isolates of Staphylococcus aureus. While the model rat skin injuries treated with HtrA healed slower than in the case of chymotrypsin, their recovery was significantly faster in compare with pure storage buffer. On the other hand, the number of bacterial CFUs on the injuries treated with HtrA solution was reduced five times, similarly to chymotrypsin treated ones. By the way, the resident microflora content of protease-treated and control wounds remained almost similar within 4 days, with Enterococcus faecalis and Staphylococcus epidermidis being the main resident microflora after the treatment. To the 8th day, the amount of staphylococcal cells was drastically reduced on HtrA- and chymotrypsin treated wound surfaces, confirming that both proteases provide wound cleaning

Keywords

Serine proteases, Bacillus subtilis, HtrA

The name of the journal

BioNanoScience

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=138243&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Akhmetov Nafis Fanisovich	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Bogachev Mikhail Igorevich	ru_RU
dc.contributor.author	Shigapova Zukhra Rashidovna	ru_RU
dc.contributor.author	Baltin Maksim Eduardovich	ru_RU
dc.contributor.author	Sharafutdinov Irshad Sultanovich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Sharafutdinov I. HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuries / I. Sharafutdinov, Z. Shigapova, M. Baltin, N. Akhmetov, M. Bogachev, A. Kayumov // BioNanoScience. – 2016 – V.6(4). – P.564-567.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=138243&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	The gene of serine protease HtrA from Bacillus subtilis was cloned and recombinant protein was overexpressed in E.coli and purified. The recombinant HtrA efficiently repressed in vitro the biofilm formation by clinical isolates of Staphylococcus aureus. While the model rat skin injuries treated with HtrA healed slower than in the case of chymotrypsin, their recovery was significantly faster in compare with pure storage buffer. On the other hand, the number of bacterial CFUs on the injuries treated with HtrA solution was reduced five times, similarly to chymotrypsin treated ones. By the way, the resident microflora content of protease-treated and control wounds remained almost similar within 4 days, with Enterococcus faecalis and Staphylococcus epidermidis being the main resident microflora after the treatment. To the 8th day, the amount of staphylococcal cells was drastically reduced on HtrA- and chymotrypsin treated wound surfaces, confirming that both proteases provide wound cleaning	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Serine proteases	ru_RU
dc.subject	Bacillus subtilis	ru_RU
dc.subject	HtrA	ru_RU
dc.title	HtrA protease from Bacillus subtilis suppresses the bacterial fouling of the rat skin injuriesa	ru_RU
dc.type	Articles in international journals and collections	ru_RU