KFU. Card publication. Visualizing trimming dependence of biodistribution and kinetics with homo- and heterogeneous N-glycoclusters on fluorescent albumin

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VISUALIZING TRIMMING DEPENDENCE OF BIODISTRIBUTION AND KINETICS WITH HOMO- AND HETEROGENEOUS N-GLYCOCLUSTERS ON FLUORESCENT ALBUMIN

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Kurbangalieva Almira Rafaelovna, author

Tanaka Kacunori , author

Bibliographic description in the original language

Ogura A. Visualizing trimming dependence of biodistribution and kinetics with homo- and heterogeneous N-glycoclusters on fluorescent albumin / A. Ogura, T. Tahara, S. Nozaki, K. Morimoto, Y. Kizuka, S. Kitazume, M. Hara, S. Kojima, H. Onoe, A. Kurbangalieva, N. Taniguchi, Y. Watanabe, K. Tanaka // Sci. Rep. – 2016. – V. 6. – Art. � 21797.

Annotation

A series of N-glycans, each sequentially trimmed from biantennary sialoglycans, were homo- or heterogeneously clustered efficiently on fluorescent albumin using a method that combined strainpromoted alkyne-azide cyclization and 6π-azaelectrocyclization. Noninvasive in vivo kinetics and dissection analysis revealed, for the first time, a glycan-dependent shift from urinary to gall bladder excretion mediated by sequential trimming of non-reducing end sialic acids. N-glycoalbumins that were trimmed further, in particular, GlcNAc- and hybrid biantennary-terminated congeners, were selectively taken up by sinusoidal endothelial and stellate cells in the liver, which are critical for diagnosis and treatment of liver fibrillation. Our glycocluster strategy can not only reveal the previously unexplored extracellular functions of N-glycan trimming, but will be classified as the newly emerging glycoprobes for diagnostic and therapeutic applications.

Keywords

N-glycan, in vivo molecular imaging

The name of the journal

SCIENTIFIC REPORTS

URL

http://www.nature.com/articles/srep21797

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=135635&p_lang=2

Resource files

File name	Size (MB)	Format
Ogura_SciRep_2016_6_21797.pdf	1,65	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Kurbangalieva Almira Rafaelovna	ru_RU
dc.contributor.author	Tanaka Kacunori	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Ogura A. Visualizing trimming dependence of biodistribution and kinetics with homo- and heterogeneous N-glycoclusters on fluorescent albumin / A. Ogura, T. Tahara, S. Nozaki, K. Morimoto, Y. Kizuka, S. Kitazume, M. Hara, S. Kojima, H. Onoe, A. Kurbangalieva, N. Taniguchi, Y. Watanabe, K. Tanaka // Sci. Rep. – 2016. – V. 6. – Art. � 21797.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=135635&p_lang=2	ru_RU
dc.description.abstract	SCIENTIFIC REPORTS	ru_RU
dc.description.abstract	A series of N-glycans, each sequentially trimmed from biantennary sialoglycans, were homo- or heterogeneously clustered efficiently on fluorescent albumin using a method that combined strainpromoted alkyne-azide cyclization and 6π-azaelectrocyclization. Noninvasive in vivo kinetics and dissection analysis revealed, for the first time, a glycan-dependent shift from urinary to gall bladder excretion mediated by sequential trimming of non-reducing end sialic acids. N-glycoalbumins that were trimmed further, in particular, GlcNAc- and hybrid biantennary-terminated congeners, were selectively taken up by sinusoidal endothelial and stellate cells in the liver, which are critical for diagnosis and treatment of liver fibrillation. Our glycocluster strategy can not only reveal the previously unexplored extracellular functions of N-glycan trimming, but will be classified as the newly emerging glycoprobes for diagnostic and therapeutic applications.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	N-glycan	ru_RU
dc.subject	in vivo molecular imaging	ru_RU
dc.title	Visualizing trimming dependence of biodistribution and kinetics with homo- and heterogeneous N-glycoclusters on fluorescent albumin	ru_RU
dc.type	Articles in international journals and collections	ru_RU