KFU. Card publication. Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

KAZAN
FEDERAL UNIVERSITY

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FULL-GENOME STUDY OF GENE EXPRESSION IN LUMBAR SPINAL CORD OF MICE AFTER 30-DAY SPACE FLIGHT ON BION-M1 BIOSATELLITE

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Gusev Oleg Aleksandrovich, author

Petrov Konstantin Aleksandrovich, author

Rizvanov Albert Anatolevich, author

Tyapkina Oksana Viktorovna, author

Bibliographic description in the original language

Islamov R.R. Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite /R.R. Islamov, O.A. Gusev, A. Tanabe, M. Terada, O.V. Tyapkina, K.A. Petrov, A.A. Rizvanov, I.B. Kozlovskaya, E.E. Nikolskiy, A.I. Grigorjev // Acta Astronautica, Volume 122, 2016, Pages 231-236

Annotation

Experiments were conducted on male mice of the C57BL/6J for full genome study of gene expression. The animals were divided into two groups.The 'flight group' mice that had spent 30 days in space and the 'control group' mice that stayed on Earth. The spinal cord of the 'flight' group mice as extracted 14h following the biosatellite's landing. The spinal cord of the control group mice was extracted at the same time.Then the lumbar spinal cord was frozen in liquid nitrogen and kept until the beginning of the study at -80?C. Quality control of the feature was performed using the settings recommended by Agilent Technologies. Signal background was subtracted and the signal intensity of each gene was globally normalized using locally weighted scatterplot smoothing. The analysis suggests that various functional groups of genes in different types of cells in spinal cord may be involved in the development of HLS and some changes in gene expression certainly happened in motor neurons innervating

Keywords

Bion-M1 biosatellite

The name of the journal

Acta Astronautica

URL

http://www.scopus.com/inward/record.url?eid=2-s2.0-84958982369&partnerID=40&md5=72cba688a263281a3d8766aeb4ca8e42

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=135547&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gusev Oleg Aleksandrovich	ru_RU
dc.contributor.author	Petrov Konstantin Aleksandrovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Tyapkina Oksana Viktorovna	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Islamov R.R. Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite /R.R. Islamov, O.A. Gusev, A. Tanabe, M. Terada, O.V. Tyapkina, K.A. Petrov, A.A. Rizvanov, I.B. Kozlovskaya, E.E. Nikolskiy, A.I. Grigorjev // Acta Astronautica, Volume 122, 2016, Pages 231-236	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=135547&p_lang=2	ru_RU
dc.description.abstract	Acta Astronautica	ru_RU
dc.description.abstract	Experiments were conducted on male mice of the C57BL/6J for full genome study of gene expression. The animals were divided into two groups.The 'flight group' mice that had spent 30 days in space and the 'control group' mice that stayed on Earth. The spinal cord of the 'flight' group mice as extracted 14h following the biosatellite's landing. The spinal cord of the control group mice was extracted at the same time.Then the lumbar spinal cord was frozen in liquid nitrogen and kept until the beginning of the study at -80?C. Quality control of the feature was performed using the settings recommended by Agilent Technologies. Signal background was subtracted and the signal intensity of each gene was globally normalized using locally weighted scatterplot smoothing. The analysis suggests that various functional groups of genes in different types of cells in spinal cord may be involved in the development of HLS and some changes in gene expression certainly happened in motor neurons innervating	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite	ru_RU
dc.type	Articles in international journals and collections	ru_RU