KFU. Card publication. Amperometric immunosensor for nonylphenol determination based on peroxidase indicating reaction

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

AMPEROMETRIC IMMUNOSENSOR FOR NONYLPHENOL DETERMINATION BASED ON PEROXIDASE INDICATING REACTION

Form of presentation

Articles in international journals and collections

Year of publication

2006

��

��

Authors, employees of KFU

Budnikov German Konstantinovich, author

Evtyugin Gennadiy Arturovich, author

Bibliographic description in the original language

Evtugyn G.A. Amperometric immunosensor for nonylphenol determination based on peroxidase indicating reaction [Text] / G.A. Evtugyn, S.A. Eremin, S.A., R.P. Shaljamova, A.R. Ismagilova, H.C. Budnikov // Biosens. Bioelectron. - 2006. - V.22. - P.56-62.

Annotation

Novel immunosensor for nonylphenol (NP) determination has been developed by immobilization of specific antibodies together with horseradish peroxidase on the surface of carbon screen-printed electrode. The signal of the immunosensor is generated by the involvement of NP accumulated in the peroxidase oxidation of mediator (Methylene Blue, hydroquinone or iodide). This results in the increase of the signal recorded by linear-sweep voltammetry. The sensitivity of the detection depends on the nature of mediator, its concentration and incubation period. Cross-selectivity of the response toward readily oxidized phenolic compounds has been determined. The immunosensor developed makes it possible to detect from 20 μg L-1 to 44 mg L-1 of NP with detection limit 10 μg L-1 of NP.

Keywords

Screen-printed electrode, immunosensor, nonylphenol determination

The name of the journal

BIOSENS BIOELECTRON

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-33746482756&partnerID=40&md5=ea8d348187cf0a5c1a1a461b249b2af7

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=135389&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Budnikov German Konstantinovich	ru_RU
dc.contributor.author	Budnikov German Konstantinovich	ru_RU
dc.contributor.author	Evtyugin Gennadiy Arturovich	ru_RU
dc.date.accessioned	2006-01-01T00:00:00Z	ru_RU
dc.date.available	2006-01-01T00:00:00Z	ru_RU
dc.date.issued	2006	ru_RU
dc.identifier.citation	Evtugyn G.A. Amperometric immunosensor for nonylphenol determination based on peroxidase indicating reaction [Text] / G.A. Evtugyn, S.A. Eremin, S.A., R.P. Shaljamova, A.R. Ismagilova, H.C. Budnikov // Biosens. Bioelectron. - 2006. - V.22. - P.56-62.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=135389&p_lang=2	ru_RU
dc.description.abstract	BIOSENS BIOELECTRON	ru_RU
dc.description.abstract	Novel immunosensor for nonylphenol (NP) determination has been developed by immobilization of specific antibodies together with horseradish peroxidase on the surface of carbon screen-printed electrode. The signal of the immunosensor is generated by the involvement of NP accumulated in the peroxidase oxidation of mediator (Methylene Blue, hydroquinone or iodide). This results in the increase of the signal recorded by linear-sweep voltammetry. The sensitivity of the detection depends on the nature of mediator, its concentration and incubation period. Cross-selectivity of the response toward readily oxidized phenolic compounds has been determined. The immunosensor developed makes it possible to detect from 20 μg L-1 to 44 mg L-1 of NP with detection limit 10 μg L-1 of NP.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Screen-printed electrode	ru_RU
dc.subject	immunosensor	ru_RU
dc.subject	nonylphenol determination	ru_RU
dc.title	Amperometric immunosensor for nonylphenol determination based on peroxidase indicating reaction	ru_RU
dc.type	Articles in international journals and collections	ru_RU