KFU. Card publication. Heterologous expression of Bacillus ginsengihumi phytase gene

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

HETEROLOGOUS EXPRESSION OF BACILLUS GINSENGIHUMI PHYTASE GENE

Form of presentation

Articles in international journals and collections

Year of publication

2015

Язык

английский

Authors, employees of KFU

Akhmetova Alina Ildusovna, author

Balaban Nelli Pavlovna, author

Suleymanova Aliya Damirovna, author

Toymenceva Anna Aleksandrovna, author

Sharipova Margarita Rashidovna, author

Authors, students KFU

Ilyukhina Darya Leonidovna, author

Bibliographic description in the original language

A. Akhmetova. Heterologous expression of Bacillus ginsengihumi phytase gene / A. Akhmetova, A. Suleimanova, A. Toymentseva, N. Balaban, D. Iljukhina, M. Sharipova // Research Journal of Pharmaceutical, Biological and Chemical Science.- 2015.- 6(4).-P. 117-122

Annotation

Phytate is the most abundant reservoir of phosphorus, which can potentially be used by microorganisms, plants and animals. Microbial phytases, capable of hydrolysing insoluble phytates, can be used as animal feed additives. In the present study, we isolated new phytase-producing bacteria - Bacillus ginsengihumi M2.11, which was identified using Microbial Identification System and 16S rRNA sequencing. We first cloned and sequenced the 1149 bp open reading frame encoding Bacillus ginsengihumi M2.11 phytase into the expression vector pET-46 Ek/LIC by the LIC-cloning method (cloning without ligase). The recombinant E.coli strain expressing Bacillus ginsengihumi M2.11 phytase was obtained. The molecular weight of phytase was 41 kDa

Keywords

phytase, cloning, Bacillus, expression

The name of the journal

Research Journal of Pharmaceutical, Biological and Chemical Sciences

URL

http://www.rjpbcs.com/pdf/2015_6%284%29/%5B19%5D.pdf

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=125891&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Akhmetova Alina Ildusovna	ru_RU
dc.contributor.author	Balaban Nelli Pavlovna	ru_RU
dc.contributor.author	Suleymanova Aliya Damirovna	ru_RU
dc.contributor.author	Toymenceva Anna Aleksandrovna	ru_RU
dc.contributor.author	Sharipova Margarita Rashidovna	ru_RU
dc.contributor.author	Ilyukhina Darya Leonidovna	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	A. Akhmetova. Heterologous expression of Bacillus ginsengihumi phytase gene / A. Akhmetova, A. Suleimanova, A. Toymentseva, N. Balaban, D. Iljukhina, M. Sharipova // Research Journal of Pharmaceutical, Biological and Chemical Science.- 2015.- 6(4).-P. 117-122	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=125891&p_lang=2	ru_RU
dc.description.abstract	Research Journal of Pharmaceutical, Biological and Chemical Sciences	ru_RU
dc.description.abstract	Phytate is the most abundant reservoir of phosphorus, which can potentially be used by microorganisms, plants and animals. Microbial phytases, capable of hydrolysing insoluble phytates, can be used as animal feed additives. In the present study, we isolated new phytase-producing bacteria - Bacillus ginsengihumi M2.11, which was identified using Microbial Identification System and 16S rRNA sequencing. We first cloned and sequenced the 1149 bp open reading frame encoding Bacillus ginsengihumi M2.11 phytase into the expression vector pET-46 Ek/LIC by the LIC-cloning method (cloning without ligase). The recombinant E.coli strain expressing Bacillus ginsengihumi M2.11 phytase was obtained. The molecular weight of phytase was 41 kDa	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	phytase	ru_RU
dc.subject	cloning	ru_RU
dc.subject	Bacillus	ru_RU
dc.subject	expression	ru_RU
dc.title	Heterologous expression of Bacillus ginsengihumi phytase gene	ru_RU
dc.type	Articles in international journals and collections	ru_RU