KFU. Card publication. An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene

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AN IMPROVED GENE EXPRESSION SYSTEM TO GENERATE TRANSGENIC ARABIDOPSIS THALIANA PLANTS HARBORING A BACILLUS GINSENGIHUMI PHYTASE GENE

Form of presentation

Articles in international journals and collections

Year of publication

2015

Authors, employees of KFU

Valeeva Liya Rashitovna, author

Nyamsuren Chuluunceceg ., author

Shakirov Evgeniy Vitalevich, author

Sharipova Margarita Rashidovna, author

Authors, postgraduates KFU

Valeeva Liya Rashitovna, postgraduate kfu

Bibliographic description in the original language

Nyamsuren, Ch. An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene / Ch. Nyamsuren, L. R Valeeva, M. R Sharipova, E. V Shakirov // RJPBCS. ? 2015. ? V.6(4). - P.18-24.

Annotation

We constructed a new vector system for heterologous gene expression in Arabidopsis thaliana. The construct contains a codon-optimized sequence encoding Bacillus ginsengihumi phytase behind an inducible plant-specific promoter for expression in root epithelial cells. The new vector introduced into the plant A. thaliana by Agrobacterium mediated transformation. We obtained several generations of transgenic A. thaliana plants with integrated Bacillus ginsengihumi phytase gene, as well as with an empty vector as a negative control. We tested several transgenic plants harboring the phyCg construct under the control of phosphate-starvation inducible Pht1;2 promoter and show that the phyCg gene is expressed at the mRNA level. Further characterization of these lines of plants will help us to design an improved transgenic strategy for the development of a root-specific heterologous system for the expression of bacterial phytases in plants.

Keywords

Bacillus phytase, Pht1;2 promoter, Arabidopsis thaliana

The name of the journal

Research Journal of Pharmaceutical, Biological and Chemical Sciences

URL

http://www.rjpbcs.com/pdf/2015_6(4)/[4].pdf

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=125196&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Valeeva Liya Rashitovna	ru_RU
dc.contributor.author	Nyamsuren Chuluunceceg .	ru_RU
dc.contributor.author	Shakirov Evgeniy Vitalevich	ru_RU
dc.contributor.author	Sharipova Margarita Rashidovna	ru_RU
dc.contributor.author	Valeeva Liya Rashitovna	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	Nyamsuren, Ch. An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene / Ch. Nyamsuren, L. R Valeeva, M. R Sharipova, E. V Shakirov // RJPBCS. ? 2015. ? V.6(4). - P.18-24.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=125196&p_lang=2	ru_RU
dc.description.abstract	Research Journal of Pharmaceutical, Biological and Chemical Sciences	ru_RU
dc.description.abstract	We constructed a new vector system for heterologous gene expression in Arabidopsis thaliana. The construct contains a codon-optimized sequence encoding Bacillus ginsengihumi phytase behind an inducible plant-specific promoter for expression in root epithelial cells. The new vector introduced into the plant A. thaliana by Agrobacterium mediated transformation. We obtained several generations of transgenic A. thaliana plants with integrated Bacillus ginsengihumi phytase gene, as well as with an empty vector as a negative control. We tested several transgenic plants harboring the phyCg construct under the control of phosphate-starvation inducible Pht1;2 promoter and show that the phyCg gene is expressed at the mRNA level. Further characterization of these lines of plants will help us to design an improved transgenic strategy for the development of a root-specific heterologous system for the expression of bacterial phytases in plants.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bacillus phytase	ru_RU
dc.subject	Pht1;2 promoter	ru_RU
dc.subject	Arabidopsis thaliana	ru_RU
dc.title	An Improved Gene Expression System to Generate Transgenic Arabidopsis Thaliana Plants Harboring a Bacillus Ginsengihumi Phytase Gene	ru_RU
dc.type	Articles in international journals and collections	ru_RU