KFU. Card publication. High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase.

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FEDERAL UNIVERSITY

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HIGH-RESOLUTION STRUCTURE OF A MEMBRANE PROTEIN TRANSFERRED FROM AMPHIPOL TO A LIPIDIC MESOPHASE.

Form of presentation

Articles in Russian journals and collections

Year of publication

2014

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Authors, employees of KFU

Sincov Mikhail Yurevich, author

Other authors

Polovinkin Vitaly -, author

Bibliographic description in the original language

Polovinkin V, High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase. / Gushchin I, Sintsov M, Round E, Balandin T, Chervakov P, Schevchenko V, Utrobin P, Popov A, orshchevskiy V, Mishin A, Kuklin A, Willbold D,Chupin V, Popot JL, Gordeliy V. // J Membr Biol. 2014 Oct;247(9-10):997-1004. doi: 10.1007/s00232-014-9700-x. Epub 2014 Sep 6.

Annotation

Journal of Membrane Biology

Keywords

Amphipol, Membrane protein, crystallization, Bacteriorhodopsin, Monoolein, In meso crystallization

The name of the journal

Journal of Membrane Biology

URL

http://link.springer.com/article/10.1007/s00232-014-9700-x/fulltext.html

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=124594&p_lang=2

Resource files

File name	Size (MB)	Format
art%3A10.1007%2Fs00232_014_9700_x.pdf	0,75	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sincov Mikhail Yurevich	ru_RU
dc.contributor.author	Polovinkin Vitaly -	ru_RU
dc.date.accessioned	2014-01-01T00:00:00Z	ru_RU
dc.date.available	2014-01-01T00:00:00Z	ru_RU
dc.date.issued	2014	ru_RU
dc.identifier.citation	Polovinkin V, High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase. / Gushchin I, Sintsov M, Round E, Balandin T, Chervakov P, Schevchenko V, Utrobin P, Popov A, orshchevskiy V, Mishin A, Kuklin A, Willbold D,Chupin V, Popot JL, Gordeliy V. // J Membr Biol. 2014 Oct;247(9-10):997-1004. doi: 10.1007/s00232-014-9700-x. Epub 2014 Sep 6.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=124594&p_lang=2	ru_RU
dc.description.abstract	Journal of Membrane Biology	ru_RU
dc.description.abstract	Journal of Membrane Biology	ru_RU
dc.description.abstract	Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs solubilized in APols would be of high importance for structural biology. However, despite considerable efforts, it is still not clear whether MP/APol complexes can form well-ordered crystals suitable for X-ray crystallography. In the present work, we show that an APol-trapped MP can be crystallized in meso. Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with a lipidic mesophase, and crystallization was induced by adding a precipitant. The crystals diffract beyond 2 Å. The structure of BR was solved to 2 Å and found to be indistinguishable from previous structures obtained after transfer from detergent solutions. We suggest the proposed protocol of in meso crystallization to be generally applicable to APol-trapped MPs.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Amphipol	ru_RU
dc.subject	Membrane protein	ru_RU
dc.subject	crystallization	ru_RU
dc.subject	Bacteriorhodopsin	ru_RU
dc.subject	Monoolein	ru_RU
dc.subject	In meso crystallization	ru_RU
dc.title	High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase.	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU