KFU. Card publication. Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine

KAZAN
FEDERAL UNIVERSITY

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- 中文
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LUMINESCENT SILICA NANOPARTICLES FOR SENSING ACETYLCHOLINESTERASE-CATALYZED HYDROLYSIS OF ACETYLCHOLINE

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Zueva Irina Vladimirovna, author

Masson Patrik Ivon Moris , author

Mustafina Asiya Rafaelevna, author

Mukhametshina Alsu Rustemovna, author

Petrov Konstantin Aleksandrovich, author

Authors, postgraduates KFU

Mukhametshina Alsu Rustemovna, postgraduate kfu

Bibliographic description in the original language

Mukhametshina A.R. Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine / A. R. Mukhametshina, S. V. Fedorenko, I. V. Zueva, K. A. Petrov, P. Masson, I. R. Nizameev, A. R. Mustafina, O.G. Sinyashin // Biosensors and Bioelectronics. - 2016. - V. 77. - P. 871-878.

Annotation

This work highlights the H-function of Tb(III)-doped silica nanoparticles in aqueous solutions of acetic acid as a route to sense acetylcholinesterase-catalyzed hydrolysis of acetylcholine (ACh). The H-function results from H+-induced quenching of Tb(III)-centered luminescence due to protonation of Tb(III) complexes located close to silica/water interface. The H-function can be turned on/switched off by the concentration of complexes within core or nanoparticle shell zones, by the silica surface decoration and adsorption of both organic and inorganic cations on silica surface. Results indicate the optimal synthetic procedure for making nanoparticles capable of sensing acetic acid produced by enzymatic hydrolysis of acetylcholine. The H-function of nanoparticles was determined at various concentrations of ACh and AChE. The measurements show experimental conditions for fitting the H-function to Michaelis-Menten kinetics. Results confirm that reliable fluorescent monitoring AChE-catalyzed

Keywords

Acetylcholinesterase, Acetylcholine, Enzymatic hydrolysis, Silica nanoparticles, Luminescence, Tb(III) complex

The name of the journal

Biosensors and Bioelectronics

URL

http://www.sciencedirect.com/science/article/pii/S0956566315305200

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=122103&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Zueva Irina Vladimirovna	ru_RU
dc.contributor.author	Masson Patrik Ivon Moris	ru_RU
dc.contributor.author	Mustafina Asiya Rafaelevna	ru_RU
dc.contributor.author	Mukhametshina Alsu Rustemovna	ru_RU
dc.contributor.author	Petrov Konstantin Aleksandrovich	ru_RU
dc.contributor.author	Mukhametshina Alsu Rustemovna	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Mukhametshina A.R. Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine / A. R. Mukhametshina, S. V. Fedorenko, I. V. Zueva, K. A. Petrov, P. Masson, I. R. Nizameev, A. R. Mustafina, O.G. Sinyashin // Biosensors and Bioelectronics. - 2016. - V. 77. - P. 871-878.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=122103&p_lang=2	ru_RU
dc.description.abstract	Biosensors and Bioelectronics	ru_RU
dc.description.abstract	This work highlights the H-function of Tb(III)-doped silica nanoparticles in aqueous solutions of acetic acid as a route to sense acetylcholinesterase-catalyzed hydrolysis of acetylcholine (ACh). The H-function results from H+-induced quenching of Tb(III)-centered luminescence due to protonation of Tb(III) complexes located close to silica/water interface. The H-function can be turned on/switched off by the concentration of complexes within core or nanoparticle shell zones, by the silica surface decoration and adsorption of both organic and inorganic cations on silica surface. Results indicate the optimal synthetic procedure for making nanoparticles capable of sensing acetic acid produced by enzymatic hydrolysis of acetylcholine. The H-function of nanoparticles was determined at various concentrations of ACh and AChE. The measurements show experimental conditions for fitting the H-function to Michaelis-Menten kinetics. Results confirm that reliable fluorescent monitoring AChE-catalyzed	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Acetylcholinesterase	ru_RU
dc.subject	Acetylcholine	ru_RU
dc.subject	Enzymatic hydrolysis	ru_RU
dc.subject	Silica nanoparticles	ru_RU
dc.subject	Luminescence	ru_RU
dc.subject	Tb(III) complex	ru_RU
dc.title	Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine	ru_RU
dc.type	Articles in international journals and collections	ru_RU