KFU. Card publication. Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red

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FEDERAL UNIVERSITY

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LABEL-FREE ELECTROCHEMICAL APTASENSOR FOR CYTOCHROME C DETECTION USING PILLAR[5]ARENE BEARING NEUTRAL RED

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Evtyugin Vladimir Gennadevich, author

Evtyugin Gennadiy Arturovich, author

Osin Yuriy Nikolaevich, author

Stoykov Ivan Ivanovich, author

Shurpik Dmitriy Nikolaevich, author

Bibliographic description in the original language

Stepanova, V.B. Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red [Text] / V.B.Stepanova, D.N.Shurpik, V.G.Evtugyn, I.I.Stoikov, G.A.Evtugyn, Yu.N.Osin, T.Hianik // Sens. Actuators B.- 2016. - V.225. - P.57-65.

Annotation

Novel electrochemical aptasensor toward cytochrome c (Cyt c) has been developed on the base of glassy carbon electrode (GCE) modified with electropolymerized neutral red (Poly-NR) and decacarboxylated pillar[5]arene (P[5]A-COOH) bearing terminal neutral red (NR) and aminated aptamer specific to Cyt c. Addition of Cyt c resulted in decrease of the cathodic peak current of NR on cyclic voltammogram due to suppression of the electron exchange between reduced and oxidized NR forms in the surface layer. The implementation of Cyt c in the surface layer was confirmed by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). Depending on the content of the surface layer and assembling protocol, the limits of detection (LODs) varied from 0.02 to 1.0 nM and linear range of concentrations was within three orders of magnitude. Interfering influence of some proteins and polyethylene glycol was characterized.

Keywords

DNA aptamer, cytochrome c, electrochemical sensor, poly(neutral red), pillar[5]arene

The name of the journal

Sensors and Actuators, B: Chemical

URL

http://www.scopus.com/inward/record.url?eid=2-s2.0-84947575990&partnerID=40&md5=f9cb7437ad86907d0cc1152af33b1c9f

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=121478&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Evtyugin Vladimir Gennadevich	ru_RU
dc.contributor.author	Evtyugin Gennadiy Arturovich	ru_RU
dc.contributor.author	Osin Yuriy Nikolaevich	ru_RU
dc.contributor.author	Stoykov Ivan Ivanovich	ru_RU
dc.contributor.author	Shurpik Dmitriy Nikolaevich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Stepanova, V.B. Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red [Text] / V.B.Stepanova, D.N.Shurpik, V.G.Evtugyn, I.I.Stoikov, G.A.Evtugyn, Yu.N.Osin, T.Hianik // Sens. Actuators B.- 2016. - V.225. - P.57-65.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=121478&p_lang=2	ru_RU
dc.description.abstract	Sensors and Actuators, B: Chemical	ru_RU
dc.description.abstract	Novel electrochemical aptasensor toward cytochrome c (Cyt c) has been developed on the base of glassy carbon electrode (GCE) modified with electropolymerized neutral red (Poly-NR) and decacarboxylated pillar[5]arene (P[5]A-COOH) bearing terminal neutral red (NR) and aminated aptamer specific to Cyt c. Addition of Cyt c resulted in decrease of the cathodic peak current of NR on cyclic voltammogram due to suppression of the electron exchange between reduced and oxidized NR forms in the surface layer. The implementation of Cyt c in the surface layer was confirmed by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). Depending on the content of the surface layer and assembling protocol, the limits of detection (LODs) varied from 0.02 to 1.0 nM and linear range of concentrations was within three orders of magnitude. Interfering influence of some proteins and polyethylene glycol was characterized.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	DNA aptamer	ru_RU
dc.subject	cytochrome c	ru_RU
dc.subject	electrochemical sensor	ru_RU
dc.subject	poly(neutral red)	ru_RU
dc.subject	pillar[5]arene	ru_RU
dc.title	Label-free electrochemical aptasensor for cytochrome c detection using pillar[5]arene bearing neutral red	ru_RU
dc.type	Articles in international journals and collections	ru_RU