KFU. Card publication. MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1

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MNSOD DOWNREGULATION INDUCED BY EXTREMELY LOW 0.1 MGY SINGLE AND FRACTIONATED X-RAYS AND MICROGRAVITY TREATMENT IN HUMAN NEUROBLASTOMA CELL LINE, NB-1

Form of presentation

Articles in Russian journals and collections

Year of publication

2015

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Authors, employees of KFU

Gusev Oleg Aleksandrovich, author

Bibliographic description in the original language

MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1 HP Indo et. al. Journal of clinical biochemistry and nutrition 57 (2), 98, 2015

Annotation

A human neuroblastoma cell line, NB-1, was treated with 24 h of microgravity simulation by clinostat, or irradiated with extremely small X-ray doses of 0.1 or 1.0 mGy using single and 10 times fractionation regimes with 1 and 2 h time-intervals. A quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) examination was performed for apoptosis related factors (BAX, CYTC, APAF1, VDAC1?3, CASP3, CASP8, CASP9 P53, AIF, ANT1 and 2, BCL2, MnSOD, autophagy related BECN and necrosis related CYP-40. The qRT-PCR results revealed that microgravity did not result in significant changes except for a upregulation of proapoptotic VDAC2, and downregulations of proapoptotic CASP9 and antiapoptotic MnSOD. After 0.1 mGy fractionation irradiation, there was increased expression of proapoptotic APAF1 and downregulation of proapoptotic CYTC, VDAC2, VDAC3, CASP8, AIF, ANT1, and ANT2, as well as an increase in expression of antiapoptotic BCL2. There was also a decrease in MnSOD express

Keywords

MnSOD, microgravity

The name of the journal

Journal of Clinical Biochemistry and Nutrition

URL

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566025/

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=121019&p_lang=2

Resource files

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jcbn15_20.pdf	0,18	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gusev Oleg Aleksandrovich	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1 HP Indo et. al. Journal of clinical biochemistry and nutrition 57 (2), 98, 2015	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=121019&p_lang=2	ru_RU
dc.description.abstract	Journal of Clinical Biochemistry and Nutrition	ru_RU
dc.description.abstract	A human neuroblastoma cell line, NB-1, was treated with 24 h of microgravity simulation by clinostat, or irradiated with extremely small X-ray doses of 0.1 or 1.0 mGy using single and 10 times fractionation regimes with 1 and 2 h time-intervals. A quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) examination was performed for apoptosis related factors (BAX, CYTC, APAF1, VDAC1?3, CASP3, CASP8, CASP9 P53, AIF, ANT1 and 2, BCL2, MnSOD, autophagy related BECN and necrosis related CYP-40. The qRT-PCR results revealed that microgravity did not result in significant changes except for a upregulation of proapoptotic VDAC2, and downregulations of proapoptotic CASP9 and antiapoptotic MnSOD. After 0.1 mGy fractionation irradiation, there was increased expression of proapoptotic APAF1 and downregulation of proapoptotic CYTC, VDAC2, VDAC3, CASP8, AIF, ANT1, and ANT2, as well as an increase in expression of antiapoptotic BCL2. There was also a decrease in MnSOD express	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	MnSOD	ru_RU
dc.subject	microgravity	ru_RU
dc.title	MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU