KFU. Card publication. Carbon monoxide modulates electrical activity of murine myocardium via cGMP-dependent mechanisms

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CARBON MONOXIDE MODULATES ELECTRICAL ACTIVITY OF MURINE MYOCARDIUM VIA CGMP-DEPENDENT MECHANISMS

Form of presentation

Articles in international journals and collections

Year of publication

2015

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Authors, employees of KFU

Sitdikova Guzel Faritovna, author

Other authors

Denis V. Abramochkin Olga P. Konovalova Andre Kamkin, author

Bibliographic description in the original language

Abramochkin, D.V.,Konovalova, O.P., Kamkin, A.,Sitdikova, G.F. Carbon monoxide modulates electrical activity of murine myocardium via cGMP-dependent mechanisms Journal of Physiology and Biochemistry Volume 71, Issue 1, 2015, P.107-119

Annotation

Carbon monoxide (CO) is critical in cell signaling, and inhalation of gaseous CO can impact cardiovascular physiology. We have investigated electrophysiological effects of CO and their potential cGMP-dependent mechanism in isolated preparations of murine myocardium. The standard microelectrode technique was used to record myocardial action potentials (APs). Exogenous CO (0.96×10−4? 4.8×10−4 M) decreased AP duration in atrial and ventricular tissue and accelerated pacemaking activity in sinoatrial node. Inhibitors of heme oxygenases (zinc and tin protoporphyrin IX), which are responsible for endogenous CO production, induced the opposite effects. Inhibitor of soluble guanylate cyclase (sGC), ODQ (10−5 M) halved CO-induced AP shortening, while sGC activator azosidnone (10−5 M-3×10−4 M) and cGMP analog BrcGMP (3×10− 4 M) induced the same effects as CO. To see if CO effects are attributed to differential regulation of phosphodiesterase 2 (PDE2) and 3 (PDE3), we used inhibitors of these enz

Keywords

Carbon monoxide . Heart . Action potential . Guanylate cyclase . cGMP. cAMP

The name of the journal

Journal of Physiology and Biochemistry

URL

http://link.springer.com/article/10.1007%2Fs13105-015-0387-y

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=112258&p_lang=2

Resource files

File name	Size (MB)	Format
CO_JPB.pdf	2,07	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Sitdikova Guzel Faritovna	ru_RU
dc.contributor.author	Denis V. Abramochkin Olga P. Konovalova Andre Kamkin	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	Abramochkin, D.V.,Konovalova, O.P., Kamkin, A.,Sitdikova, G.F. Carbon monoxide modulates electrical activity of murine myocardium via cGMP-dependent mechanisms Journal of Physiology and Biochemistry Volume 71, Issue 1, 2015, P.107-119	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=112258&p_lang=2	ru_RU
dc.description.abstract	Journal of Physiology and Biochemistry	ru_RU
dc.description.abstract	Carbon monoxide (CO) is critical in cell signaling, and inhalation of gaseous CO can impact cardiovascular physiology. We have investigated electrophysiological effects of CO and their potential cGMP-dependent mechanism in isolated preparations of murine myocardium. The standard microelectrode technique was used to record myocardial action potentials (APs). Exogenous CO (0.96×10−4? 4.8×10−4 M) decreased AP duration in atrial and ventricular tissue and accelerated pacemaking activity in sinoatrial node. Inhibitors of heme oxygenases (zinc and tin protoporphyrin IX), which are responsible for endogenous CO production, induced the opposite effects. Inhibitor of soluble guanylate cyclase (sGC), ODQ (10−5 M) halved CO-induced AP shortening, while sGC activator azosidnone (10−5 M-3×10−4 M) and cGMP analog BrcGMP (3×10− 4 M) induced the same effects as CO. To see if CO effects are attributed to differential regulation of phosphodiesterase 2 (PDE2) and 3 (PDE3), we used inhibitors of these enz	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Carbon monoxide modulates electrical activity of murine myocardium via cGMP-dependent mechanisms	ru_RU
dc.type	Articles in international journals and collections	ru_RU