24 December 2019
17th article this year

Article title: Force interactions between Yersiniae lipopolysaccharides and monoclonal antibodies: An optical tweezers study

Journal name: Journal of Biomechanics, 13 November 2019

Authors: Ilya Konyshev, Andrey Byvalov, Boris Ananchenko, Rawil Fakhrullin, Anna Danilushkina, Lyubov Dudina

Russian scientists found nonspecific force interactions between Yersinia lipopolysaccharides and monoclonal antibodies

Scientists from Kirov, in collaboration with colleagues from Kazan and Syktyvkar, in a joint article report a force spectroscopic study of the interactions between the lipopolysaccharides of two species of the Yersinia and complementary (or heterologous) monoclonal antibodies. The experimental data were obtained using the optical tweezers method.

As a model system, a “sensitized polystyrene microsphere - a substrate of sensitized glass” was used. The histograms revealed nonspecific interactions in areas with low amplitude, mainly due to the physicochemical properties of the abiotic surface and specific interactions in complementary pairs of “antigen-antibodies” in areas with high amplitude (100-120 pN). The results of the study can be used to detect rupture forces in other molecular pairs. The ability to determine the intermolecular strength between Yersinia lipopolysaccharides and complementary monoclonal antibodies can be an effective approach for assessing the relative affinity of monoclonal antibodies, which is necessary to select one type or another of antibodies to develop a test system or immunosensors. On the other hand, the application of these methodological approaches can be useful for understanding the interaction between lipopolysaccharides and complementary monoclonal antibodies, as well as for elucidating the general mechanisms of immunity and pathogenesis of yersiniosis infections.