Brain damage led to decrease of nitric oxide content in the hippocampus of rats: Investigation by EPR spectroscopy

G.G. Yafarova a,b, V.V. Andrianov a,b, S.G. Pashkevich c, Ju.P. Stukach c, M.O. Dosina c, T.Kh. Bogodvid a,d, V.A. Kulchitsky c, Kh.L. Gainutdinov a,b

a Kazan Federal University, Kazan, 420008 Russia

b Zavoisky Physical-Technical Institute, FRC Kazan Scientific Center of Russian Academy of Sciences, Kazan, 420034 Russia

c Institute of Physiology of National Academy of Sciences of Belarus, Minsk, 220072 Republic of Belarus

d Volga Region State Academy of Physical Culture, Kazan, 420010 Russia

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Abstract

Direct measurements of the content of nitric oxide (NO) by the method of spectroscopy of electron paramagnetic resonance were performed. It was found that NO production in hippocampus tissues decreased to 46% after 5 h of ischemic stroke as compared to the control group and remained at the same level after 72 h. Following the hemorrhagic stroke, the level of NO production in the hippocampus decreased to 32% after 5 h of hemorrhagic stroke of the control level. After 72 hours of hemorrhagic stroke NO production was higher than after 5 h and amounted to 48% of the level of intact animals.

Keywords: nitric oxide, ischemic brain stroke, hemorrhagic brain stroke, hippocampus, electron paramagnetic resonance

Acknowledgments. The study was supported by the Russian Foundation for Basic Research (project no. 18-515-00003) and by the Belarusian Republican Foundation for Fundamental Research (project no. B18R-227).

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Received

May 17, 2018

Yafarova Guzel Gul’usovna, Candidate of Biological Sciences, Assistant Lecturer of Department of Human and Animal Physiology; Senior Researcher of Laboratory of Spin Physics and Spin Chemistry

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

Zavoisky Physical-Technical Institute, FRC Kazan Scientific Center of RAS

ul. Sibirsky tract, 10/7 Kazan, 420029, Russia

E-mail: gusadila@mail.ru

Andrianov Vyatcheslav Vadimovich, Candidate of Biological Sciences, Senior Researcher of Rehabilitation in Movement Disorders OpenLab; Senior Researcher of Laboratory of Spin Physics and Spin Chemistry

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

Zavoisky Physical-Technical Institute, FRC Kazan Scientific Center of RAS

ul. Sibirsky tract, 10/7 Kazan, 420029, Russia

E-mail: slava_snail@yahoo.com

Pashkevich Svetlana Georgievna, Candidate of Biological Sciences, Head of Laboratory of Neurophysiology

Institute of Physiology, National Academy of Sciences of Belarus

ul. Akademicheskaya , 28, Minsk 220072 Republic of Belarus

E-mail: skypasht@mail.ru

Stukach Julia Pavlovna, Researcher of Laboratory of Neurophysiology

Institute of Physiology, National Academy of Sciences of Belarus

ul. Akademicheskaya , 28, Minsk 220072 Republic of Belarus

E-mail: stukachyulya@gmail.com

Dosina Margarita Olegovna, Candidate of Biological Sciences, Senior Researcher of Laboratory of Neurophysiology

Institute of Physiology, National Academy of Sciences of Belarus

ul. Akademicheskaya, 28, Minsk 220072 Republic of Belarus

E-mail: pochta_margo@mail.ru

Bogodvid Tatiana Khalilovna, Doctor of Biological Sciences, Senior Researcher of Rehabilitation in Movement Disorders OpenLab; Associate Professor of Department of Medico-Biological Sciences

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

Volga Region State Academy of Physical Culture, Sport and Tourism

Universiade Village, 35, Kazan, 420010, Russia

E-mail: tat-gain@mail.ru

Kulchitsky Vladimir Adamovich, Doctor of Medical Sciences, Professor, Academician of National Academy of Sciences of Belarus, Assistant Director

Institute of Physiology, National Academy of Sciences of Belarus

ul. Akademicheskaya, 28, Minsk 220072 Republic of Belarus

E-mail: vladi@fizio.bas-net.by

Gainutdinov Khalil Latypovich, Doctor of Biological Sciences, Professor, Leading Researcher of Rehabilitation in Movement Disorders OpenLab; Leading researcher of Laboratory of Spin Physics and Spin Chemistry

Kazan Federal University

ul. Kremlevskaya, 18, Kazan, 420008 Russia

Zavoisky Physical-Technical Institute, FRC Kazan Scientific Center of RAS

ul. Sibirsky tract, 10/7 Kazan, 420029, Russia

E-mail: gusadila@mail.ru

 

For citation: Yafarova G.G., Andrianov V.V., Pashkevich S.G., Stukach Ju.P., Dosina M.O., Bogodvid T.Kh., Kulchitsky V.A., Gainutdinov Kh.L. Brain damage led to decrease of nitric oxide content in the hippocampus of rats: Investigation by EPR spectroscopy. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2018, vol. 160, no. 4, pp. 654–662.

Для цитирования: Yafarova G.G., Andrianov V.V.,  Pashkevich S.G.,  Stukach Ju.P.,  Dosina M.O., Bogodvid T.Kh., Kulchitsky V.A., Gainutdinov Kh.L. Brain damage led to decrease of nitric oxide content in the hippocampus of rats: Investigation by EPR spectroscopy // Учен. зап. Казан. ун-та. Сер. Естеств. науки. – 2018. – Т. 160, кн. 4. – С. 654–662.

 

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