L.G. Shaidarova*, A.V. Gedmina**, V.D. Demina***, I.A. Chelnokova****, H.C. Budnikov*****
Kazan Federal University, Kazan, 420008 Russia
E-mail: *LarisaShaidarova@mail.ru, **Anna.Gedmina@kpfu.ru, ***firstname.lastname@example.org, ****Irina.Chelnokova@kpfu.ru, *****Herman.Budnikov@kpfu.ru
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Electrodeposited gold and Nafion-gold composite on the surface of glassy carbon electrodes (GCE) have shown electrocatalytic activity during guanine oxidation. In comparison with the unmodified electrode, decreasing of the oxidation potential by 100 mV and increasing of the current of organic compound oxidation have been observed. When the Nafion (NF) film is applied to the surface of the glassy carbon electrode with electrodeposited gold, a five-fold increase of guanine oxidation current has been achieved compared to its oxidation on the modified electrode without the NF film.
Conditions have been found for electrodeposition of gold on the surface of the glassy carbon electrode, including that one covered with the NF film, as well as for registration of the maximum catalytic current on these electrodes. Linear dependence of the electrocatalytic response of the modified electrode from the guanine concentration has been observed in the range from 510–6 to 510–3 mol×L–1 (for Au GCE) and from 510–7 to 510–3 mol×L–1 (for NF-Au GCE).
Keywords: chemically modified electrodes, voltammetric determination of guanine
Acknowledgments. This study was funded by the subsidy allocated as part of the state program for increasing the competitiveness of Kazan Federal University among the world's leading centers of science and education.
Fig. 1. Cyclic voltamperograms obtained on the Au GCE in the solution of 0.01 M H2SO4 (a) and in solutions with different pH values (b): 1– 2.0; 2 – 6.86; 3– 10.
Fig. 2. Cyclic voltamperograms obtained on the Au GCE (a) in the absence (1) and in the presence (2) of guanine (with c = 510–3 М) at the background of the sulfuric acid solution with pH 2.0; dependence of the maximum oxidation current on the concentration of guanine (b); the diagram showing dependencies of the catalytic effect during guanine oxidation on the time of gold deposition on the GCE.
Fig. 3. Dependence of the maximum guanine oxidation current on the CME with gold deposit on the potential superposition rate in logarithmical coordinates (a), on the square root of the potential superposition rate (b), dependence of the maximum guanine oxidation potential on the potential superposition rate (c).
Fig. 4. Dependence of guanine oxidation current on the NF-Au GCE on the concentration of Nafion (a) and the time of gold deposition (b).
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For citation: Shaidarova L.G., Gedmina A.V., Demina V.D., Chelnokova I.A., Budnikov H.C. Voltammetric determination and amperometric detection of guanine on the electrode modified by gold deposit and nafion film. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 2016, vol. 158, no. 3, pp. 369–380. (In Russian)
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