| Форма представления | Статьи в зарубежных журналах и сборниках |
| Год публикации | 2016 |
| Язык | английский |
|
Абдуллин Тимур Илдарович, автор
Гафуров Марат Ревгерович, автор
Камалов Марат Ильясович, автор
Ризванов Альберт Анатольевич, автор
Часов Виталий Васильевич, автор
|
| Библиографическое описание на языке оригинала |
Chasov V.V. EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3 / V. Chasov, A. Rodionov, M. Gafurov, M. Kamalov,T. Abdullin, S. Zaitsev, A. Rizvanov // BioNanoSci. (2016). DOI: 10.1007/s12668-016-0294-x |
| Аннотация |
Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy. |
| Ключевые слова |
Nitroxide spin label, Electron paramagnetic resonance, Recombinant human histone, H1.3, Plasmid DNA, Biosensing |
| Название журнала |
BioNanoScience
|
| URL |
https://doi:10.1007/s12668-016-0294-x |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=145712 |
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Абдуллин Тимур Илдарович |
ru_RU |
| dc.contributor.author |
Гафуров Марат Ревгерович |
ru_RU |
| dc.contributor.author |
Камалов Марат Ильясович |
ru_RU |
| dc.contributor.author |
Ризванов Альберт Анатольевич |
ru_RU |
| dc.contributor.author |
Часов Виталий Васильевич |
ru_RU |
| dc.date.accessioned |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2016-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2016 |
ru_RU |
| dc.identifier.citation |
Chasov V.V. EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3 / V. Chasov, A. Rodionov, M. Gafurov, M. Kamalov,T. Abdullin, S. Zaitsev, A. Rizvanov // BioNanoSci. (2016). DOI: 10.1007/s12668-016-0294-x |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=145712 |
ru_RU |
| dc.description.abstract |
BioNanoScience |
ru_RU |
| dc.description.abstract |
Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Nitroxide spin label |
ru_RU |
| dc.subject |
Electron paramagnetic resonance |
ru_RU |
| dc.subject |
Recombinant human histone |
ru_RU |
| dc.subject |
H1.3 |
ru_RU |
| dc.subject |
Plasmid DNA |
ru_RU |
| dc.subject |
Biosensing |
ru_RU |
| dc.title |
EPR detection of DNA interaction with 3-carboxy-proxyl-labelled recombinant human histone H1.3 |
ru_RU |
| dc.type |
Статьи в зарубежных журналах и сборниках |
ru_RU |
|
EN 
中文 
ES 
Карта сайта
